Oryza sativa H+-ATPase (OSA) is Involved in the Regulation of Dumbbell-Shaped Guard Cells of Rice.
Oryza sativa H+-ATPase (OSA) is Involved in the Regulation of Dumbbell-Shaped Guard Cells of Rice.
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DOI：
10.1093/pcp/pcw070
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发表时间：
2016-06
影响因子：
4.9
通讯作者：
Kinoshita T
中科院分区：
文献类型：
--
作者：
Toda Y;Wang Y;Takahashi A;Kawai Y;Tada Y;Yamaji N;Feng Ma J;Ashikari M;Kinoshita T
气孔器由一对保卫细胞组成，调节叶片与大气之间的气体交换。在保卫细胞中，蓝光（BL）通过其倒数第二个苏氨酸的磷酸化激活质膜上的H+-ATP酶，介导气孔开放。虽然这种调控被认为是广泛采用的双子叶植物的肾形保卫细胞，在单子叶植物哑铃形保卫细胞的分子基础仍然不清楚。在这里，我们表明，H+-ATP酶参与哑铃形保卫细胞的调节。H ~+-ATPase激活剂fusicoccin和BL均能促进水稻气孔开放，而BL则被H ~+-ATPase抑制剂vanadate抑制。利用H ~+-ATP酶抗体，我们发现水稻H ~+-ATP酶（OSAs）倒数第二个苏氨酸的磷酸化调节和OSAs在保卫细胞质膜上的定位。有趣的是，我们鉴定了一种H+-ATP酶同工型OSA 7，其在保卫细胞中的OSA基因中优先表达，并且发现OSA 7的功能丧失导致对BL的部分不敏感性。我们的结论是，H+-ATP酶参与BL诱导的哑铃形保卫细胞在单子叶植物气孔开放。
The stomatal apparatus consists of a pair of guard cells and regulates gas exchange between the leaf and atmosphere. In guard cells, blue light (BL) activates H+-ATPase in the plasma membrane through the phosphorylation of its penultimate threonine, mediating stomatal opening. Although this regulation is thought to be widely adopted among kidney-shaped guard cells in dicots, the molecular basis underlying that of dumbbell-shaped guard cells in monocots remains unclear. Here, we show that H+-ATPases are involved in the regulation of dumbbell-shaped guard cells. Stomatal opening of rice was promoted by the H+-ATPase activator fusicoccin and by BL, and the latter was suppressed by the H+-ATPase inhibitor vanadate. Using H+-ATPase antibodies, we showed the presence of phosphoregulation of the penultimate threonine in Oryza sativa H+-ATPases (OSAs) and localization of OSAs in the plasma membrane of guard cells. Interestingly, we identified one H+-ATPase isoform, OSA7, that is preferentially expressed among the OSA genes in guard cells, and found that loss of function of OSA7 resulted in partial insensitivity to BL. We conclude that H+-ATPase is involved in BL-induced stomatal opening of dumbbell-shaped guard cells in monocotyledon species.