Dietary Fungal Glucosylceramide and Ceramide Reduce the Formation of Aberrant Crypt Foci in 1,2-Dimethylhydrazine-Treated Mice: Differences in the Role of Glucosylceramide and Ceramide
Dietary Fungal Glucosylceramide and Ceramide Reduce the Formation of Aberrant Crypt Foci in 1,2-Dimethylhydrazine-Treated Mice: Differences in the Role of Glucosylceramide and Ceramide
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DOI：
10.1021/acsfoodscitech.2c00290
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发表时间：
2023-01-02
期刊：
影响因子：
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通讯作者：
Kinoshita，Mikio
中科院分区：
文献类型：
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作者：
Yamashita，Shinji;Tsuruma，Tomoya;Kinoshita，Mikio
葡萄糖神经酰胺（GlcCer）由连接到神经酰胺（Cer）的葡萄糖部分组成。据报道，GlcCer可减轻体内肠损伤并抑制体外肠炎症。在这项研究中，为了阐明GlcCer对体内肠损伤的作用，我们比较了香菇衍生的GlcCer（真菌GlcCer）与具有真菌GlcCer的相同Cer部分的真菌Cer对异常隐窝灶（ACF）形成和结肠炎症的作用。真菌GlcCer或铈补充饮食显着抑制ACF形成在1，2-二甲基肼（DMH）处理的小鼠。膳食真菌GlcCer降低DMH诱导的炎症相关细胞因子和炎症相关蛋白的表达，而膳食真菌Cer几乎不降低它们在肠道损伤后的表达。此外，当小鼠摄入真菌GlcCer时，仅在粪便中检测到少量真菌Cer。这些结果表明，摄入的真菌GlcCer可能很难代谢成真菌Cer，并且GlcCer本身可能通过抑制肠道炎症来抑制DMH处理的小鼠中ACF的形成。
Glucosylceramide (GlcCer) is composed of a glucose moiety attached to ceramides (Cer). GlcCer was reported to reduce the intestinal impairmentsin vivoand suppressin vitrointestinal inflammation. In this study, to clarify the effect of GlcCer on intestinal impairmentsin vivo, we compared the effects of shiitake mushroom-derived GlcCer (fungal GlcCer) on the formation of aberrant crypt foci (ACF) and colon inflammation with those of fungal Cer having the same Cer moiety of the fungal GlcCer. Fungal GlcCer- or Cer-supplemented diets significantly suppressed ACF formation in 1,2-dimethylhydrazine (DMH)-treated mice. Dietary fungal GlcCer reduced the expression of inflammation-related cytokines and apoptosis-related proteins induced by DMH, whereas dietary fungal Cer hardly reduced their expression upon intestinal injuries. Furthermore, when fungal GlcCer was ingested to the mice, only a small amount of fungal Cer was detected in the feces. These results indicate that the ingested fungal GlcCer might be hardly metabolized to fungal Cer and the GlcCer itself might suppress ACF formation in DMH-treated mice by suppressing intestinal inflammation.