Crosslinked Poly(N-Isopropylacrylamide)-Based Microfibers as Cell Manipulation Materials with Prompt Cell Detachment.
Crosslinked Poly(N-Isopropylacrylamide)-Based Microfibers as Cell Manipulation Materials with Prompt Cell Detachment.
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DOI：
10.1002/marc.201900464
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发表时间：
2019-12
影响因子：
4.6
通讯作者：
Tomomi Konishi;Aya Mizutani Akimoto;Taihei Nishimoto;Yuki Tokura;Mizuki Tenjimbayashi;Kenta Homma;Ko Matsukawa;Taisei Kaku;Y. Hiruta;K. Nagase;H. Kanazawa;S. Shiratori
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文献类型：
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作者：
Tomomi Konishi;Aya Mizutani Akimoto;Taihei Nishimoto;Yuki Tokura;Mizuki Tenjimbayashi;Kenta Homma;Ko Matsukawa;Taisei Kaku;Y. Hiruta;K. Nagase;H. Kanazawa;S. Shiratori
刺激响应智能材料是实现下一代医疗技术的关键。其中，温度响应性聚合物聚（N-异丙基丙烯酰胺）（PNIPAAm）由于易于在生理条件下使用而受到特别关注。 PNIPAAm 接枝表面可以在没有蛋白水解酶的情况下进行温度调节的细胞粘附和分离，并且可以通过选择性细胞粘附/分离用作细胞分离材料。然而，在低温下细胞分离是有问题的，因为它需要几个小时。本研究介绍了一种新型热响应交联微纤维系统，可以大大减少细胞脱离时间。交联纤维提供与温度相关的体积变化，并在降低温度后 10 分钟内使细胞脱离，这是之前研究所需时间的六分之一。细胞的迅速脱离被认为是由纤维膨胀衍生的全新机制引起的。该系统作为新型细胞操纵系统将为下一代医疗技术做出重大贡献。
Stimuli-responsive smart materials are a key to the realization of next-generation medical technologies. Among them, the temperature-responsive polymer poly(N-isopropylacrylamide) (PNIPAAm) is attracting particular attention because it is easy to use in physiological conditions. PNIPAAm-grafted surfaces can undergo temperature-modulated cell adhesion and detachment without proteolytic enzymes, and can be used as cell-separating materials through selective cell adhesion/detachment. However, cell detachment at reduced temperatures is problematic because it takes several hours. A novel thermoresponsive crosslinked microfiber system that can greatly reduce the cell detachment time is introduced in this study. The crosslinked fibers provide temperature-dependent volume change, and enable cell detachment within 10 min of reducing the temperature, which is one-sixth of the time required in previous studies. The prompt cell detachment is thought to arise from a completely new mechanism derived from fiber swelling. This system will make a significant contribution as a novel cell manipulating system for next-generation medical technology.