A FISH-based chromosome map for the European corn borer yields insights into ancient chromosomal fusions in the silkworm
A FISH-based chromosome map for the European corn borer yields insights into ancient chromosomal fusions in the silkworm
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DOI：
10.1038/hdy.2015.72
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发表时间：
2014-12
期刊：
影响因子：
3.8
通讯作者：
Y. Yasukochi;Mizuki Ohno;F. Shibata;A. Jouraku;Ryo Nakano;Yukio Ishikawa;Ken Sahara
中科院分区：
文献类型：
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作者：
Y. Yasukochi;Mizuki Ohno;F. Shibata;A. Jouraku;Ryo Nakano;Yukio Ishikawa;Ken Sahara
鳞翅目、蝴蝶和蛾类基因组的一个显著特征是染色体组的高度保守性。最近在鳞翅目昆虫基因组测序方面取得的显著进展表明，同线基因顺序在亲缘关系较远的物种中广泛保守。鳞翅目的祖先核型被认为是n= 31;然而，研究最充分的蛾，家蚕，是n= 28，不同的研究表明，三个染色体融合事件发生在这个谱系。以确定预测的涉及B的古代融合之间的界限。利用家蚕第11、23和24号染色体，构建了欧洲玉米螟（Ostrinia nubilalis，n= 31）的荧光原位杂交（fluorescence in situ hybridization，FISH）染色体图谱。首次测定了亚洲玉米螟O. Acidacalis是O. nubilalis，和分离的细菌人工染色体和Fosmid克隆，预期使用这些序列定位于边界的候选区域。结合FISH和遗传分析，我们将候选区域缩小到40 kb-1.5 Mb，与先前基于蝴蝶Melitaea cinxia基因组的估计高度一致。没有观察到功能基因的候选区域长度的显著差异可能反映了融合事件后的进化时间。
A significant feature of the genomes of Lepidoptera, butterflies and moths, is the high conservation of chromosome organization. Recent remarkable progress in genome sequencing of Lepidoptera has revealed that syntenic gene order is extensively conserved across phylogenetically distant species. The ancestral karyotype of Lepidoptera is thought to be n= 31; however, that of the most well-studied moth, Bombyx mori, is n= 28, and diverse studies suggest that three chromosomal fusion events occurred in this lineage. To identify the boundaries between predicted ancient fusions involving B. mori chromosomes 11, 23 and 24, we constructed fluorescence in situ hybridization (FISH)-based chromosome maps of the European corn borer, Ostrinia nubilalis (n= 31). We first determined a 511 Mb genomic sequence of the Asian corn borer, O. furnacalis, a congener of O. nubilalis, and isolated bacterial artificial chromosomes and fosmid clones that were expected to localize in candidate regions for the boundaries using these sequences. Combined with FISH and genetic analysis, we narrowed down the candidate regions to 40 kb–1.5 Mb, in strong agreement with a previous estimate based on the genome of a butterfly, Melitaea cinxia. The significant difference in the lengths of the candidate regions where no functional genes were observed may reflect the evolutionary time after fusion events.