The effect of enzyme addition on anaerobic digestion of Jose Tall Wheat Grass
The effect of enzyme addition on anaerobic digestion of Jose Tall Wheat Grass
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DOI：
10.1016/j.biortech.2008.12.065
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发表时间：
2009-10-01
影响因子：
11.4
通讯作者：
McGarvey, Jeffery A.
中科院分区：
文献类型：
--
作者：
Romano, Rowena T.;Zhang, Ruihong;McGarvey, Jeffery A.
使用Jose高小麦草（小麦草）作为模型底物，研究了添加含有纤维素酶、半纤维素酶和β-葡萄糖苷酶的酶产物到厌氧消化系统中的影响。厌氧消化试验使用在50 ℃下操作的间歇反应器进行。对酶制剂在3种消化系统中的应用进行了模拟和研究：（1）单级消化器中添加酶;（2）麦草经酶预处理后进行单级厌氧消化;（3）两级消化系统的第一级（水解和酸化）添加酶。单独使用酶制剂处理小麦草时，酶制剂对小麦草的增溶效果良好。然而，当在厌氧消化系统中测试酶产物时，在沼气和甲烷产率以及挥发性固体减少方面没有显著差异。这表明接种物中存在的微生物在进行小麦草的消化中是有效的。使用16 S rRNA序列分析鉴定接种物中存在的微生物类型。不同接种物之间的序列的比较显示，普遍的操作分类单位是相似的，但酸化接种物中含有较高比例的种Thermotogae。(C)2009爱思唯尔有限公司保留所有权利。
The effects of the addition of enzyme products containing cellulase, hemicellulase, and beta-glucosidase to anaerobic digestion systems were studied using Jose Tall Wheat Grass (wheat grass) as a model substrate. Anaerobic digestion tests were performed using batch reactors operated at 50 degrees C. The application of enzyme products in three digestion configurations were simulated and investigated: (1) enzyme addition to a single-stage digester, (2) pre-treatment of wheat grass with enzymes followed by a single-stage anaerobic digestion, and (3) enzyme addition to the first stage (hydrolysis and acidification) of a two-stage digestion system. The enzyme products showed positive effects on the solubilization of wheat grass when used alone to treat the wheat grass. However, no significant differences in biogas and methane yields, and volatile solids reduction resulted when the enzyme products were tested in the anaerobic digestion systems. This reveals that the microorganisms present in the inoculum were effective in carrying out the digestion of wheat grass. The types of microorganisms present in the inoculum were identified using 16S rRNA sequence analysis. A comparison of the sequences between the different inocula revealed that the prevalent operational taxonomic units were similar, but that the acidified inoculum contained a higher percentage of the species Thermotogae. (C) 2009 Elsevier Ltd. All rights reserved.