A colorimetric gold nanoparticle sensor to interrogate biomolecular interactions in real time on a surface
A colorimetric gold nanoparticle sensor to interrogate biomolecular interactions in real time on a surface
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DOI：
10.1021/ac015657x
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发表时间：
2002-02-01
影响因子：
7.4
通讯作者：
Chilkoti, A
中科院分区：
文献类型：
--
作者：
Nath, N;Chilkoti, A
本文提出了一种新的无标记光学方法来实时研究光学透明基底表面的生物分子相互作用。该方法依赖于玻璃上自组装单层胶体金的吸光度光谱的变化，作为固定胶体表面生物分子结合的功能。使用这种方法，我们证明了无标记光学生物传感器的原理，可以在市售的紫外可见分光光度计中实时量化表面上的生物分子相互作用，并使用光学扫描仪进行比色终点分析。该分光光度传感器显示出浓度依赖性结合，对链霉亲和素的检测限为16 nM。该传感器易于制造，其性能可重复，技术要求最低，即紫外可见分光光度计或光学扫描仪的可用性，并且将能够以阵列为基础的格式实时高通量筛选生物分子相互作用。
This paper presents a new label-free optical method to study biomolecular interactions in real time at the surface of an optically transparent substrate. The method relies on the change in the absorbance spectrum of a self-assembled monolayer of colloidal gold on glass, as a function of biomolecular binding to the surface of the immobilized colloids. Using this approach, we demonstrate proof of principle of a label-free optical biosensor to quantify biomolecular interactions in real time on a surface in a commercially available UV-visible spectrophotometer and of a colorimetric end-point assay using an optical scanner. The spectrophotometric sensor shows concentration-dependent binding and a detection limit of 16 nM for streptavidin. The sensor is easy to fabricate, is reproducible in its performance, has minimal technological requirements, namely, the availability of an UV-visible spectrophotometer or an optical scanner, and will enable high-throughput screening of biomolecular interactions in real time in an array-based format.