Stimulatory effect of the sulfated polysaccharide ascophyllan on the respiratory burst in RAW264.7 macrophages
Stimulatory effect of the sulfated polysaccharide ascophyllan on the respiratory burst in RAW264.7 macrophages
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DOI：
10.1016/j.ijbiomac.2012.09.008
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发表时间：
2013-01-01
影响因子：
8.2
通讯作者：
Oda, Tatsuya
中科院分区：
文献类型：
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作者：
Wang, Yajun;Jiang, Zedong;Oda, Tatsuya
在这项研究中，我们从产生活性氧(ROS)的角度研究了天冬青多糖的生物活性。在RAW264.7细胞中，我们发现阿司匹林以浓度依赖的方式诱导ROS的产生，但呈钟形分布。免疫印迹分析表明，天冬氨酸多糖促进NADPH氧化酶胞质亚基(P67(Phox)和P47(Phox))向质膜的移位。在所测试的丝裂原活化蛋白(MAP)激酶抑制剂中，JNK抑制剂对天冬酰胺诱导的ROS产生的抑制作用最强。一贯地，在天冬青处理的RAW264.7细胞中检测到显著水平的磷酸化JNK蛋白激酶。我们的发现首次表明，阿司匹林可以通过激活NADPH氧化酶和JNK MAPK来刺激巨噬细胞产生ROS。(C)2012爱思唯尔B.V.保留所有权利。
In this study, we investigated the bioactivity of ascophyllan in terms of reactive oxygen species (ROS) generation. In RAW264.7 cells, we found that ascophyllan induced ROS generation in a concentration-dependent manner, but with bell-shaped profile. Immunoblot analysis demonstrated that ascophyllan promoted the translocation of cytosolic subunits (p67(phox) and p47(phox)) of NADPH oxidase to the plasma membrane. Among mitogen activated protein (MAP) kinase inhibitors tested, JNK inhibitor showed the most potent inhibitory effect on ascophyllan-induced ROS production. Consistently, significant level of phosphorylated JNK MAP kinase was detected in ascophyllan-treated RAW264.7 cells. Our findings suggest for the first time that ascophyllan can stimulate macrophages to produce ROS through the activations of NADPH oxidase and JNK MAP kinase. (C) 2012 Elsevier B.V. All rights reserved.