Clinical studies on bovine leukemia in Japanese black cattle. III. Serum lactate dehydrogenase activity and its isoenzyme pattern in groups of leukemic cattle and those negative or positive for antibody against bovine leukemia virus.
Clinical studies on bovine leukemia in Japanese black cattle. III. Serum lactate dehydrogenase activity and its isoenzyme pattern in groups of leukemic cattle and those negative or positive for antibody against bovine leukemia virus.
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日本黑牛牛白血病的临床研究。
DOI：
10.1292/jvms1939.42.289
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发表时间：
1980
期刊：
影响因子：
--
通讯作者：
M. Onuma
中科院分区：
文献类型：
--
作者：
K. Ishihara;T. Ohtani;H. Kitagawa;M. Onuma
用血清乳酸脱氢酶（S-LDH）测定了牛白血病病毒抗体阳性和阴性两组178头日本黑牛的血清乳酸脱氢酶（S-LDH）活性。结果，两组在任何时间检测时S-LDH活性均无显著差异。7 ~ 12个月后，经免疫扩散或补体固定试验，阴性组部分牛抗体阳性。与抗体阴性时的S-LDH活性相比，他们没有显示出任何显著的变化。随机选取19头抗体阳性牛与抗体阴性牛进行酶谱比较。结果显示，阳性牛的LDH2和LDH3活性显著升高，LDH1活性显著降低。8头白血病牛的平均S-LDH活性显著高于阳性组和阴性组，但2头白血病牛的平均S-LDH活性没有升高。此外，即使在没有增加总S-LDH活性的情况下，牛的LDH2和LDH3同工酶活性也显著增加。
Serum lactate dehydrogenase (S-LDH) activity was determined four times over a time course in 178 Japanese Black cattle of two groups which were positive and negative, respectively, for antibody against bovine leukemia virus. As a result, no significant difference in S-LDH activity was evidenced by the two groups at any time of testing. Seven to 12 months later, some cattle of the negative group were antiobdy-positive when examined by the immunodiffusion or complement fixation test. They failed to indicate any significant change in S-LDH activity, as compared with this activity shown when they were antibody-negative. A comparison was made between the zymograms of 19 antibody-positive cattle and antibody-negative cattle, both of which groups were randomly selected. They revealed significantly high LDH2 and LDH3 activity and markedly low LDH1 activity in the positive cattle. The mean S-LDH activity was significantly higher in 8 leukemic cattle than in both positive and negative groups, although no increase was found in 2 leukemic cattle. Moreover, the isoenzyme activity showed a significant increase in LDH2 and LDH3 even in cattle exhibiting no increase in total S-LDH activity.