p75NTR-Immunoreactivity in the subventricular zone of adult male rats:: Expression by cycling cells
p75NTR-Immunoreactivity in the subventricular zone of adult male rats:: Expression by cycling cells
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DOI：
10.1007/s10735-004-9609-2
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发表时间：
2004-11-01
影响因子：
3.2
通讯作者：
Calzà, L
中科院分区：
文献类型：
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作者：
Giuliani, A;D'Intino, G;Calzà, L
虽然胚胎和成人神经球中神经干细胞谱系的体外调控研究取得了很大进展，但对成人脑中神经干细胞谱系的原位作用因素知之甚少。我们报道了神经营养素低亲和力受体p75(NTR)存在于成年雄性大鼠的脑室下带(SVZ)。然后，我们鉴定了与前体细胞相关的标记(Nestin和PSA-NCAM)与生长因子受体(p75(NTR)，TrkA)的共同分布。免疫细胞化学和激光共聚焦扫描显微镜观察成年雄性大鼠的增殖相关抗原(Ki67和BrDU摄取)。用不同的单抗和多克隆抗血清研究p75(NTR)免疫反应性(IR)的分布。P75(NTR)不与胶质纤维酸性蛋白共分布。发现它与少量Nestin-IR细胞共分布。而未观察到与PSA-NCAM-IR共存。相反，p75(NTR)-IR存在于大量分裂细胞(Ki-67阳性)中，并与EGFR共同分布。为了验证p75(Ntr)与细胞死亡之间的可能联系，我们研究了p75(Ntr)-IR的共同分布与核凝集图像，如Hoechst 33258染色所示。而在SVZ内几乎没有观察到核凝聚的图像。未发现与p75(NTR)共存。在SVZ中未发现TrkA-和TrkB-IR。我们还研究了出生后第1天和第16天的p75(NTR)免疫染色，因为在这段时间段，SVZ中的增殖细胞急剧减少。P75(NTR)-IR在出生后早期未见升高。因此，P75(NTR)似乎与成年大鼠脑内SVZ的细胞周期调控有关。
While the study of in vitro regulation of neural stein cell lineage from both embryonic and adult neurospheres is greatly advanced, much less is known about factors acting in situ for neural stem cell lineage in adult brain. We reported that neurotrophin low affinity receptor p75(NTR) is present in the subventricular zone (SVZ) in adult male rats. We then characterized co-distribution of markers associated with precursor cells (nestin and PSA-NCAM) with growth factor receptors (p75(NTR), trkA. EGFr) and proliferation-associated antigens (Ki67 and BrDU-uptake) in adult male rat by immunocytochemistry and confocal laser scan microscopy. Distribution of p75(NTR) immunoreactivity (IR) was investigated using different mono- and polyclonal antisera. p75(NTR) is not co-distributed with glial fibrillary acid protein. It was found to be co-distributed with a small number of nestin-IR cells. whereas no coexistence with PSA-NCAM-IR was observed. Conversely p75(NTR)-IR was present In numerous dividing cells (Ki-67-positive) and co-distributed with EGFr. In order to verify the possible association between p75(NTR) and cell death, we investigated co-distribution of p75(NTR)-IR with nuclear condensation images, as visualized by Hoechst 33258 staining. While few images indicating nuclear condensation were observed in the SVZ. no coexistence with p75(NTR) was found. TrkA- and trkB-IR was not found in the SVZ. We also investigated p75(NTR) immunostaining on post-natal day I and day 16, because of the dramatic reduction of proliferating, cells in SVZ over this time-interval. p75(NTR)-IR was not increased in the early post-natal phase. Thus, P75(NTR) seems to be associated with cell cycle regulation in SVZ in adult rat brain.