Grapevine Rootstocks Differentially Affect the Rate of Ripening and Modulate Auxin-Related Genes in Cabernet Sauvignon Berries.
Grapevine Rootstocks Differentially Affect the Rate of Ripening and Modulate Auxin-Related Genes in Cabernet Sauvignon Berries.
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DOI：
10.3389/fpls.2016.00069
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发表时间：
2016
影响因子：
5.6
通讯作者：
Bonghi C
中科院分区：
文献类型：
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作者：
Corso M;Vannozzi A;Ziliotto F;Zouine M;Maza E;Nicolato T;Vitulo N;Meggio F;Valle G;Bouzayen M;Müller M;Munné-Bosch S;Lucchin M;Bonghi C
在现代葡萄栽培中，将商业葡萄品种嫁接到种间砧木上是一种常见的做法，以提高对许多生物和非生物胁迫的抗性。然而，利用砧木获得这些基本特性也是已知的影响葡萄果实发育和品质的因素，尽管其潜在的机制仍然鲜为人知。在葡萄浆果中，成熟的开始受到一个复杂的移动信号网络的调节，这些信号包括生长素、乙烯、脱落酸和油菜素类固醇等激素。最近，一个新的砧木，命名为M4，是基于其对水分胁迫的耐受性增强和中等活力而被选择的。本研究以商品用1103P为砧木，研究了M4对赤霞珠浆果发育的影响。生理生化指标表明，CS浆果嫁接到M4上后熟速度较快。对来自两种嫁接组合的浆果果皮和果肉的mRNA-Seq数据进行的多因素分析表明，控制生长素作用的基因(ARF和AUX/IAA)是受砧木基因型影响的主要类别之一。考虑到生长素水平严格调控这些基因的转录，我们研究了参与生长素生物合成和接合的主要基因家族的行为。分子和生化分析证实了浆果发育速度和生长素代谢调节之间的联系。此外，数据表明，与皮肤组织相比，这种现象在皮肤组织中似乎特别明显。
In modern viticulture, grafting commercial grapevine varieties on interspecific rootstocks is a common practice required for conferring resistance to many biotic and abiotic stresses. Nevertheless, the use of rootstocks to gain these essential traits is also known to impact grape berry development and quality, although the underlying mechanisms are still poorly understood. In grape berries, the onset of ripening (véraison) is regulated by a complex network of mobile signals including hormones such as auxins, ethylene, abscisic acid, and brassinosteroids. Recently, a new rootstock, designated M4, was selected based on its enhanced tolerance to water stress and medium vigor. This study investigates the effect of M4 on Cabernet Sauvignon (CS) berry development in comparison to the commercial 1103P rootstock. Physical and biochemical parameters showed that the ripening rate of CS berries is faster when grafted onto M4. A multifactorial analysis performed on mRNA-Seq data obtained from skin and pulp of berries grown in both graft combinations revealed that genes controlling auxin action (ARF and Aux/IAA) represent one of main categories affected by the rootstock genotype. Considering that the level of auxin tightly regulates the transcription of these genes, we investigated the behavior of the main gene families involved in auxin biosynthesis and conjugation. Molecular and biochemical analyses confirmed a link between the rate of berry development and the modulation of auxin metabolism. Moreover, the data indicate that this phenomenon appears to be particularly pronounced in skin tissue in comparison to the flesh.