Genome-wide high-resolution mapping of exosome substrates reveals hidden features in the Arabidopsis transcriptome
Genome-wide high-resolution mapping of exosome substrates reveals hidden features in the Arabidopsis transcriptome
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DOI：
10.1016/j.cell.2007.10.056
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发表时间：
2007-12-28
期刊：
影响因子：
64.5
通讯作者：
Belostotsky, Dmitry A.
中科院分区：
文献类型：
--
作者：
Chekanova, Julia A.;Gregory, Brian D.;Belostotsky, Dmitry A.
外泌体复合物在RNA代谢中起着核心和必要的作用。然而，对外泌体底物及其亚基的功能分析缺乏全面的研究。在这里，我们证明了与酵母和后生动物相反，植物外显体核心具有意想不到的功能可塑性，并提出了拟南芥外显体靶点的全基因组图谱。此外，我们的研究为植物中广泛的聚腺苷化和外泌体介导的RNA质量控制提供了证据，揭示了稳定结构RNA代谢的意想不到的方面，并揭示了许多新的外泌体底物。这些包括mrna的精选子集，miRNA加工中间体和数百种非编码rna，其中绝大多数以前没有被描述过，并且属于转录组的一个层，只能在抑制外泌体活性时才能看到。这些外泌体底物的首次全基因组图谱将有助于阐明真核生物转录组的新基本成分和调控机制。
The exosome complex plays a central and essential role in RNA metabolism. However, comprehensive studies of exosome substrates and functional analyses of its subunits are lacking. Here, we demonstrate that as opposed to yeast and metazoans the plant exosome core possesses an unanticipated functional plasticity and present a genome-wide atlas of Arabidopsis exosome targets. Additionally, our study provides evidence for widespread polyadenylation- and exosome-mediated RNA quality control in plants, reveals unexpected aspects of stable structural RNA metabolism, and uncovers numerous novel exosome substrates. These include a select subset of mRNAs, miRNA processing intermediates, and hundreds of noncoding RNAs, the vast majority of which have not been previously described and belong to a layer of the transcriptome that can only be visualized upon inhibition of exosome activity. These first genome-wide maps of exosome substrates will aid in illuminating new fundamental components and regulatory mechanisms of eukaryotic transcriptomes.