REDUCED OXYGENATION IN DIABETIC RAT KIDNEYS MEASURED BY T2*WEIGHTED MAGNETIC RESONANCE MICRO-IMAGING
REDUCED OXYGENATION IN DIABETIC RAT KIDNEYS MEASURED BY T2*WEIGHTED MAGNETIC RESONANCE MICRO-IMAGING
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DOI：
10.1007/978-0-387-85998-9_31
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发表时间：
2009-01-01
期刊：
影响因子：
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通讯作者：
Palm, Fredrik
中科院分区：
文献类型：
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作者：
Edlund, Jenny;Hansell, Peter;Palm, Fredrik
通过应用侵入性技术直接测量氧分压，我们报告了实验性糖尿病大鼠肾脏氧合降低的情况。然而，利用BOLD效应的非侵入性MRI技术提供了几个优点，可以在相同的动物和人类受试者中进行重复测量。在这项研究中，我们应用一种改进的单一梯度回波显微成像序列来检测糖尿病大鼠肾脏的BOLD效应，并将结果与正常血糖对照组进行比较。所有测量都是在石油灭活的成年雄性Wistar Furth大鼠身上进行的。在MRI分析前14天，用链脲佐菌素(45 mg/kg)诱发糖尿病。16个T2加权图像(B(0)=1.5T)使用射频破坏梯度回波序列，TE步长为2.6mls(TE(1)=12 ILS)，而TR(75ms)和每像素带宽(71.4 Hz)保持不变。两组外延髓皮质和外条的松动率R2*(1/T2*)相似(对照组皮质22.3±/-0.4对糖尿病组23.1+/-1.8赫兹，延髓外条24.9+/-0.4对26.4+/-1.8赫兹；两组均为4例)，而糖尿病大鼠延髓内侧纹区R2*增加(糖尿病组为26.1+/-2.4，对照组为18.8+/-1.4赫兹；n=4，P
By applying invasive techniques for direct measurements of oxygen tension, we have reported decreased kidney oxygenation in experimental diabetes in rats. However, the non-invasive MRI technique utilizing the BOLD effect provides several advantages with the possibility to perform repetitive measurements in the same animals and in human Subjects. In this study, we applied a modified single gradient echo microimaging sequence to detect the BOLD effect in kidneys of diabetic rats and compared the results to normoglycemic controls.All measurements were performed oil inactin-anaesthetized adult male Wistar Furth rats. Diabetes was induced by streptozotocin (45 mg/kg) 14 days prior to MRI-analysis. Sixteen T2*-weighted image records (B(0)=1.5 T) were performed using radiofrequency spoiled gradient echo sequence with 2.6 mls step increments of TE (TE(1)=12 ills), while TR (75 ms) and bandwidth per pixel (71.4 Hz) were kept constant. T2* maps were Computed by mono-exponential fitting of the pixel intensities.Relaxation rates R2* (1/T2*) in cortex and outer stripe of the outer medulla were similar in both groups (cortex for controls 22.3 +/- 0.4 vs. diabetics 23.1 +/- 1.8 Hz and outer stripe of outer medulla for controls 24.9 +/- 0.4 vs. diabetics 26.4 +/- 1.8 Hz; n=4 in both groups), whereas R2* was increased in the inner stripe Of the Outer medulla in diabetic rats (diabetics 26.1 +/- 2.4 vs. controls 18.8 +/- 1.4 Hz; n=4, P