A male reproduction-related Kazal-type peptidase inhibitor gene in the prawn, Macrobrachium rosenbergii:: Molecular characterization and expression patterns
A male reproduction-related Kazal-type peptidase inhibitor gene in the prawn, Macrobrachium rosenbergii:: Molecular characterization and expression patterns
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DOI：
10.1007/s10126-006-6026-4
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发表时间：
2007-01-01
影响因子：
3
通讯作者：
Yang, Wei-Jun
中科院分区：
文献类型：
--
作者：
Cao, Jun-Xia;Dai, Jie-Qiong;Yang, Wei-Jun
雄性生殖道中的肽酶抑制剂在哺乳动物中是众所周知的，其中它们在保护生殖道上皮免受蛋白水解损伤或调节受精过程中发挥作用。通过筛选富集雄性生殖道特异性转录物的消减cDNA克隆，获得了一个编码与Kazal型肽酶抑制剂（KPI）具有显著相似性的推定蛋白的克隆。这是第一次报告的无脊椎动物，其中男性生殖道特异性KPI基因已被确定和表征。该基因包含一个405 bp的开放阅读框（ORF）、一个72 bp的5'非翻译区（UTR）和一个259 bp的3'非翻译区（UTR）。概念上翻译的蛋白质由一个21个氨基酸的信号肽和一个113个氨基酸的成熟多肽组成，成熟多肽具有两个Kazal型结构域（以发现者命名）。仅在雄性生殖道中观察到显著水平的mRNA，而在测试的任何其他组织中均未检测到mRNA表达。该基因的转录在成熟过程中保持不变，但不是在幼虫后期。原位杂交结果表明，在输精管和终壶腹的分泌上皮细胞的mRNA的存在。
Peptidase inhibitors in the male reproductive tract are well known in mammals, in which they play roles in protecting the tract epithelium against proteolytic damage or in regulating the fertilization process. By screening the subtracted cDNA clones enriched for male reproductive tract-specific transcripts, one clone encoding a putative protein that showed significant similarity to Kazal-type peptidase inhibitor (KPI) was obtained. This is the first report of an invertebrate in which a male reproductive tract-specific KPI gene has been identified and characterized. The gene contains a 405-bp open reading frame (ORF), a 72 bp 5' untranslated region (UTR), and a 259 bp 3' UTR. The conceptually translated protein consisted of a 21-amino-acid signal peptide and a 113-amino-acid mature polypeptide with two Kazal-type domains (named after the discoverer). Significant levels of the mRNA were observed only in the male reproductive tract, while mRNA expression was not detected in any other tissues tested. The transcription of the gene remained constant during maturation, although not in the postlarval stage. In situ hybridization demonstrated the presence of the mRNA in the secretory epithelial cells of vas deferens and terminal ampullae.