Melatonin plays a critical role in inducing B lymphocyte proliferation of the bursa of Fabricius in broilers via monochromatic lights
Melatonin plays a critical role in inducing B lymphocyte proliferation of the bursa of Fabricius in broilers via monochromatic lights
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褪黑素在单色光诱导肉鸡法氏囊 B 淋巴细胞增殖中发挥关键作用
DOI：
10.1016/j.jphotobiol.2014.11.002
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发表时间：
2015-01-01
影响因子：
5.4
通讯作者：
Chen, Yaoxing
中科院分区：
文献类型：
--
作者：
Li, Jian;Cao, Jing;Chen, Yaoxing
为研究褪黑素在单色光诱导的法氏囊B淋巴细胞增殖中的作用，将360只新孵肉鸡（包括完整组、假手术组和去松果体组）暴露在发光二极管系统的蓝光（BL）、绿光（GL）、红光（RL）和白光（WL）下14d。体内和体外研究均表明，在血浆褪黑素水平改变的各组中，GL完整组肉鸡法氏囊内增殖细胞核抗原（PCNA）免疫反应淋巴细胞百分比和淋巴细胞对脂多糖的增殖反应最高。超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GSH-Px）活性和总抗氧化能力（T-AOC）最高，丙二醛（MDA）含量和诱导型一氧化氮合酶（iNOS）表达量显著降低。松果体切除术后，各光处理组SOD、GSH-Px和T-AOC水平均显著降低，MDA含量和iNOS表达均显著升高。体外注射外源性褪黑素（250 pg/mL）也显著增强了法氏囊B淋巴细胞的增殖。上述结果提示，GL光照可有效提高肉鸡法氏囊抗氧化能力，促进B淋巴细胞增殖，其机制可能与增强褪黑素分泌有关。(C) 2014 Elsevier B.V.版权所有
To study the role of melatonin in the monochromatic light-induced B lymphocyte proliferation of bursa, a total of 360 newly hatched broilers, including intact, sham-operated, and pinealectomized groups, were exposed to blue light (BL), green light (GL), red light (RL) and white light (WL) from a light-emitting diode system for 14d. Both in vivo and in vitro studies showed that the percentage of proliferating cell nuclear antigen (PCNA)-immunoreactive lymphocytes and the lymphocyte proliferation in response to lipopolysaccharide in the bursa of broilers in the GL intact group was the highest values among the different intact groups with altered plasma melatonin levels. Additionally, the superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activities and total antioxidant capability (T-AOC) were the highest, whereas malondialdehyde (MDA) content and inducible nitric oxide synthase (iNOS) expression were significantly decreased in the GL intact group. After pinealectomy, the levels of SOD, GSH-Px and T-AOC decreased remarkably in the various light-treatment groups, whereas the MDA content and iNOS expression significantly increased. The administration of exogenous melatonin (250 pg/mL) in vitro also significantly enhanced the bursal B lymphocyte proliferation. These findings suggest that GL illumination effectively elevates the antioxidative capacity to promote B lymphocyte proliferation of bursa in young broilers, which might depend on enhanced melatonin secretion. (C) 2014 Elsevier B.V. All rights reserved.