Engineering tissue tubes using novel multilayered scaffolds in the rat peritoneal cavity
Engineering tissue tubes using novel multilayered scaffolds in the rat peritoneal cavity
复制标题
DOI：
10.1002/jbm.a.31781
复制
发表时间：
2008-12-01
影响因子：
4.9
通讯作者：
Cooper-White, Justin J.
中科院分区：
文献类型：
--
作者：
Cao, Yang;Zhang, Bing;Cooper-White, Justin J.
我们的目标是开发新的支架工程组织管平滑肌样细胞自体移植。采用热致相分离法制备了直径为100 μ m的聚乳酸管形支架。通过逐层沉积技术，使用各种生物分子对支架进行表面改性，并植入大鼠的腹膜腔中。植入后3周的支架组织学分析显示，在其外表面上完全发育的组织囊，在整个内部空间存在巨噬细胞样细胞。在基质胶中涂覆的表面支持最强的细胞反应，而弹性蛋白、胶原蛋白I、胶原蛋白III或壳聚糖最外层的多层涂层显示出最低水平的细胞相互作用。虽然胶囊厚度和存在或不存在的细胞化层的内表面和外表面的支架上的差异进行了观察，这些生物分子涂层都不能克服异物反应的腹腔内，即使在存在的非吸附性HA底涂层。(C)2008 Wiley Periodicals，Inc. J Biomed Mater Res 87A：719-727，2008
Our aim was to develop novel scaffolds to engineer tissue tubes of smooth muscle-like cells for autologous grafting. Small diameter tubular poly(lactic acid) scaffolds with randomly distributed, interconnected pores up to 100 pm were produced using a thermally induced phase separation method. The scaffolds were surface modified using various biomolecules via a layer-by-layer deposition technique, and implanted in the peritoneal cavities of rats. Histological analysis of scaffolds 3 weeks after implantation showed fully-developed tissue capsules on their outer surfaces, with macrophage-like cells present throughout the internal spaces. Surfaces coated in Matrigel supported the strongest cellular response whereas multi-layer coatings with elastin, collagen I, collagen III, or chitosan outermost showed the lowest levels of cellular interaction. Although differences in capsule thickness and the presence or absence of cellularized layers on the inside and outside surfaces of the scaffolds were observed, none of these biomolecule coatings was able to overcome the foreign body response within the peritoneal cavity, even in the presence of a nonadsorptive HA undercoat. (C) 2008 Wiley Periodicals, Inc. J Biomed Mater Res 87A: 719-727, 2008