Map-based cloning and characterization of the novel yellow-green leaf gene ys83 in rice (Oryza sativa)
Map-based cloning and characterization of the novel yellow-green leaf gene ys83 in rice (Oryza sativa)
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水稻新型黄绿叶基因 ys83 的图位克隆和表征
DOI：
10.1016/j.plaphy.2016.11.007
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发表时间：
2017-02-01
影响因子：
6.5
通讯作者：
Deng, Xiaojian
中科院分区：
文献类型：
--
作者：
Ma, Xiaozhi;Sun, Xiaoqiu;Deng, Xiaojian
水稻叶片颜色突变体的研究非常广泛，目前已经鉴定出许多相应的基因。然而，叶片颜色突变机制是多种多样的，仍需要通过鉴定新基因来进一步研究。在本文中，我们从水稻（Oryza sativa）中分离出一个叶色突变体ys83。该突变体在苗期表现为黄绿色叶片表型，分蘖后期逐渐转变为浅绿色叶片。黄叶中光合色素含量显著降低，叶绿体发育迟缓。突变表型由水稻2号染色体短臂上一个核基因的隐性突变控制。基于图谱的克隆和测序分析表明，该候选基因为YS83 (LOC_Os02g05890)，编码一个含有165个氨基酸残基的蛋白。YS83基因在广泛的组织中表达，其编码的蛋白以叶绿体为靶点。在突变体中，基因YS83编码序列发生T-to-A替换，导致其编码产物过早翻译。通过引入野生型基因，ys83突变体恢复到正常的绿叶表型。综上所述，我们成功鉴定了一个新的黄绿叶基因YS83。单株生产穗数和每穗颖花数仅减少6.7%和7.6%，结实率和千粒重（种子大小）未受显著影响，因此叶色突变基因ys83可作为杂交水稻商品化生产的性状标记基因。(C) 2016年由Elsevier Masson SAS出版。
Leaf-color mutants have been extensively studied in rice, and many corresponding genes have been identified up to now. However, leaf-color mutation mechanisms are diverse and still need further research through identification of novel genes. In the present paper, we isolated a leaf-color mutant, ys83, in rice (Oryza sativa). The mutant displayed a yellow-green leaf phenotype at seedling stage, and then slowly turned into light-green leaf from late tillering stage. In its yellow leaves, photosynthetic pigment contents significantly decreased and the chloroplast development was retarded. The mutant phenotype was controlled by a recessive mutation in a nuclear gene on the short arm of rice chromosome 2. Map-based cloning and sequencing analysis suggested that the candidate gene was YS83 (LOC_Os02g05890) encoding a protein containing 165 amino acid residues. Gene YS83 was expressed in a wide range of tissues, and its encoded protein was targeted to the chloroplast. In the mutant, a T-to-A substitution occurred in coding sequence of gene YS83, which caused a premature translation of its encoded product. By introduction of the wild-type gene, the ys83 mutant recovered to normal green-leaf phenotype. Taken together, we successfully identified a novel yellow-green leaf gene YS83. In addition, number of productive panicles per plant and number of spikelets per panicle only reduced by 6.7% and 7.6%, respectively, meanwhile its seed setting rate and 1000-grain weight (seed size) were not significantly affected in the mutant, so leaf-color mutant gene ys83 could be used as a trait marker gene in commercial hybrid rice production. (C) 2016 Published by Elsevier Masson SAS.