Oxidative stress, glutathione status, sirtuin and cellular stress response in type 2 diabetes
Oxidative stress, glutathione status, sirtuin and cellular stress response in type 2 diabetes
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DOI：
10.1016/j.bbadis.2011.12.003
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发表时间：
2012-05-01
影响因子：
6.2
通讯作者：
Castellino, P.
中科院分区：
文献类型：
--
作者：
Calabrese, V.;Cornelius, C.;Castellino, P.
氧化应激在2型糖尿病及其并发症的发病机制中起主要作用。作为这种增加的氧化状态的结果，发生细胞适应性反应，需要功能性伴侣、抗氧化剂产生和蛋白质降解。本研究旨在评估2型糖尿病患者和年龄匹配的健康受试者的全身氧化应激和细胞应激反应。已经通过测量血浆中还原型和氧化型谷胱甘肽以及血浆和淋巴细胞中的戊糖苷、蛋白羰基脂质氧化产物4-羟基-2-壬烯醛和F2-异前列烷来评价全身性氧化应激，而热休克蛋白（HSP）HO-1、Hsp 72、Sirtuin-1、已经测量了Sirtuin-2和硫氧还蛋白还原酶-1（TrxR-1）以评估全身细胞应激反应。与对照组相比，2型糖尿病患者的血浆GSH/GSSG显著降低，与戊糖苷、F2-异前列腺素、羰基化合物和HNE水平升高相关。此外，淋巴细胞HO-1、Hsp 70、Trx和TrxR-1水平也明显高于正常对照组（P
Oxidative stress has been suggested to play a main role in the pathogenesis of type 2 diabetes mellitus and its complications. As a consequence of this increased oxidative status a cellular adaptive response occurs requiring functional chaperones, antioxidant production and protein degradation. This study was designed to evaluate systemic oxidative stress and cellular stress response in patients suffering from type 2 diabetes and in age-matched healthy subjects. Systemic oxidative stress has been evaluated by measuring plasma reduced and oxidized glutathione, as well as pentosidine, protein carbonyls lipid oxidation products 4-hydroxy-2-nonenal and F2-isoprostanes in plasma, and lymphocytes, whereas the lymphocyte levels of the heat shock proteins (HSP) HO-1, Hsp72, Sirtuin-1, Sirtuin-2 and thioredoxin reductase-1 (TrxR-1) have been measured to evaluate the systemic cellular stress response. Plasma GSH/GSSG showed a significant decrease in type 2 diabetes as compared to control group, associated with increased pentosidine, F2-isoprostanes, carbonyls and HNE levels. In addition, lymphocyte levels of HO-1, Hsp70, Trx and TrxR-1 (P