Protective effects of zinc salts on TPA-induced hepatic and brain lipid peroxidation, glutathione depletion, DNA damage and peritoneal macrophage activation in mice
Protective effects of zinc salts on TPA-induced hepatic and brain lipid peroxidation, glutathione depletion, DNA damage and peritoneal macrophage activation in mice
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DOI：
10.1016/s0306-3623(97)00072-4
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发表时间：
1998-01-01
期刊：
影响因子：
--
通讯作者：
Stohs, SJ
中科院分区：
文献类型：
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作者：
Bagchi, D;Vuchetich, PJ;Stohs, SJ
1. L 蛋氨酸锌、DL 蛋氨酸锌、硫酸锌、葡萄糖酸锌、L 蛋氨酸、DL 蛋氨酸和维生素 E 琥珀酸酯 (VES) 对 12-O-十四烷酰佛波醇-13-乙酸酯 (TPA) 诱导的肝和脑组织中的脂质过氧化、DNA 断裂和谷胱甘肽消耗以及活性氧产生的比较保护能力评估腹膜巨噬细胞。另外，给小鼠喂食缺锌饮食5周，并用TPA和/或L-蛋氨酸锌或DL-蛋氨酸锌进行治疗，并进行了类似的研究。2．缺锌饮食会引起肝、脑组织以及腹腔巨噬细胞的氧化应激，脂质过氧化、DNA 断裂、谷胱甘肽消耗和活性氧产生显着增强。3．用 L-蛋氨酸锌、DL-蛋氨酸锌和 VES 治疗小鼠可减少 TPA 诱导的活性氧产生，与对照值相比，腹膜巨噬细胞的化学发光分别显着降低约 45%、31% 和 47%，细胞色素 c 分别显着降低约 54%、35% 和 41%。肝脏和大脑脂质过氧化、DNA 断裂和谷胱甘肽消耗也观察到类似的结果。4．锌盐和抗氧化剂可有效防止 TPA 引起的氧化损伤。 L-蛋氨酸锌提供了最好的保护。 (C) 1998 爱思唯尔科学公司。
1. The comparative protective abilities of zinc L methionine, zinc DL methionine, zinc sulfate, zinc gluconate, L methionine, DL methionine, and vitamin E succinate (VES) on 12-O-tetradecanoylphorbol-13-acetate (TPA) induced lipid peroxidation, DNA fragmentation, and glutathione depletion in the hepatic and brain tissues, and production of reactive oxygen species by peritoneal macrophages were assessed. In addition, mice were fed a zinc deficient diet for 5 weeks, and treated with TPA and/or zinc L methionine or zinc DL-methionine and similar studies were conducted.2. The zinc-deficient diet induced oxidative stress in the hepatic and brain tissues as well as in the peritoneal macrophages as evidenced by significantly enhanced lipid peroxidation, DNA fragmentation, glutathione depletion, and production of reactive oxygen species.3. Treatment of mice with zinc L-methionine, zinc DL-methionine, and VES decreased TPA-induced reactive oxygen species production as evidenced by significant decreases in chemiluminescence in peritoneal macrophages by approximately 45%, 31%, and 47%, respectively, and cytochrome c reduction by approximately 54%, 35%, and 41%, respectively, as compared with control values. Similar results were observed with liver and brain lipid peroxidation, DNA fragmentation, and glutathione depletion.4. Zinc salts and antioxidants provided significant protection against TPA-induced oxidative damage. Zinc L-methionine provided the best protection. (C) 1998 Elsevier Science Inc.