Viscoelastic properties of human mesenchymally-derived stem cells and primary osteoblasts, chondrocytes, and adipocytes
Viscoelastic properties of human mesenchymally-derived stem cells and primary osteoblasts, chondrocytes, and adipocytes
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DOI：
10.1016/j.jbiomech.2007.06.019
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发表时间：
2008-01-01
影响因子：
2.4
通讯作者：
Guilak, Farshid
中科院分区：
文献类型：
--
作者：
Darling, Eric M.;Topel, Matthew;Guilak, Farshid
单细胞的机械特性在调节细胞-基质相互作用中发挥着重要作用，可能影响力转导过程。最近的研究还表明，细胞机械特性可能提供细胞表型的新生物标记或“生物标记”，反映疾病、分化或细胞转化中发生的特定变化。近年来特别令人感兴趣的是此类生物标志物的鉴定，这些生物标志物可用于确定干细胞的特定表型特征，从而将干细胞与原代分化细胞分开。本研究的目的是确定间充质谱系的三种原代细胞类型（软骨细胞、成骨细胞和脂肪细胞）的弹性和粘弹性特性，并检验原代分化细胞与成体干细胞（脂肪来源或骨髓来源的间充质干细胞）相比表现出不同机械特性的假设。在粘附、展开的构型中，软骨细胞、成骨细胞和脂肪细胞都表现出显着不同的机械特性，成骨细胞比软骨细胞更硬，并且都比脂肪细胞更硬。脂肪干细胞和间充质干细胞表现出彼此相似的特性，但在机械上与原代细胞不同，特别是在比较弹性模量与松弛模量的比率时。这些发现将有助于更准确地模拟间充质组织中的细胞机械环境，这有助于描述损伤阈值和疾病进展，甚至确定机械负荷对组织工程工作的影响。此外，干细胞独特的机械特性的鉴定可能会导致更成功的分选程序，以丰富多能祖细胞群。 (c) 2007 Elsevier Ltd. 保留所有权利。
The mechanical properties of single cells play important roles in regulating cell-matrix interactions, potentially influencing the process of mechanotransduction. Recent studies also suggest that cellular mechanical properties may provide novel biological markers, or "biomarkers," of cell phenotype, reflecting specific changes that occur with disease, differentiation, or cellular transformation. Of particular interest in recent years has been the identification of such biomarkers that can be used to determine specific phenotypic characteristics of stem cells that separate them from primary, differentiated cells. The goal of this study was to determine the elastic and viscoelastic properties of three primary cell types of mesenchymal lineage (chondrocytes, osteoblasts, and adipocytes) and to test the hypothesis that primary differentiated cells exhibit distinct mechanical properties compared to adult stem cells (adipose-derived or bone marrow-derived mesenchymal stem cells). In an adherent, spread configuration, chondrocytes, osteoblasts, and adipocytes all exhibited significantly different mechanical properties, with osteoblasts being stiffer than chondrocytes and both being stiffer than adipocytes. Adipose-derived and mesenchymal stem cells exhibited similar properties to each other, but were mechanically distinct from primary cells, particularly when comparing a ratio of elastic to relaxed moduli. These findings will help more accurately model the cellular mechanical environment in mesenchymal tissues, which could assist in describing injury thresholds and disease progression or even determining the influence of mechanical loading for tissue engineering efforts. Furthermore, the identification of mechanical properties distinct to stem cells could result in more successful sorting procedures to enrich multipotent progenitor cell populations. (c) 2007 Elsevier Ltd. All rights reserved.