Regulation of mitochondrial adenine nucleotide content in newborn rabbit liver.
Regulation of mitochondrial adenine nucleotide content in newborn rabbit liver.
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新生兔肝脏线粒体腺嘌呤核苷酸含量的调节。
DOI：
10.1152/ajpendo.1987.253.5.e530
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
Aprille,JR
中科院分区：
文献类型：
--
作者：
Tullson,PC;Aprille,JR
本研究探讨了出生后代谢和激素的变化和伴随的线粒体腺嘌呤核苷酸含量（ATP + ADP + AMP）在兔肝脏中的快速增加之间的关系。从组织丙酮酸和乳酸值计算的胞质NAD+/NADH浓度比在出生后1 h内线性增加6.6倍。线粒体NAD+/NADH浓度比，计算从组织乙酰乙酸和β-羟基丁酸值，出生后30分钟增加了28倍。NAD+/NADH的这些变化表明，组织氧合发生迅速，氧供应，而不是底物供应是限制线粒体呼吸在出生后立即。出生后2小时内发生的线粒体腺嘌呤核苷酸含量的正常增加被缺氧（5%O2）抑制。胰高血糖素刺激出生后线粒体腺嘌呤核苷酸的增加，但与缺氧相结合没有效果。葡萄糖和生长抑素注射抑制线粒体腺嘌呤核苷酸的增加，并增加胰岛素胰高血糖素的比例。异丙肾上腺素或双丁酰cAMP刺激，但普萘洛尔没有抑制，正常的线粒体腺嘌呤核苷酸含量的增加。酚妥拉明没有刺激出生后腺嘌呤核苷酸的积累。总之，结果表明，胰岛素与胰高血糖素的比例可能是最重要的激素调节腺嘌呤核苷酸进入线粒体基质的快速recompartmentation和组织氧合是严格允许这种激素的作用在出生后的第一个2小时。
This study examined the relationship between postnatal metabolic and hormonal changes and the accompanying rapid increase in mitochondrial adenine nucleotide content (ATP + ADP + AMP) in rabbit liver. The cytosolic NAD+/NADH concentration ratio, calculated from tissue pyruvate and lactate values, increased linearly 6.6-fold during the 1st postnatal h. The mitochondrial NAD+/NADH concentration ratio, calculated from tissue acetoacetate and beta-hydroxybutyrate values, increased 28-fold by 30 min postnatal. These changes in NAD+/NADH suggest that tissue oxygenation occurs rapidly and that oxygen supply rather than substrate supply is limiting for mitochondrial respiration in the immediate postnatal period. The normal increase in mitochondrial adenine nucleotide content that occurs within 2 h after birth was inhibited by hypoxia (5% O2). Glucagon stimulated the postnatal increase in mitochondrial adenine nucleotides but had no effect in combination with hypoxia. Both glucose and somatostatin injections inhibited the increase in mitochondrial adenine nucleotides and increased the insulin-to-glucagon ratio. Isoproterenol or dibutyryl cAMP stimulated, but propranolol did not inhibit, the normal increase in mitochondrial adenine nucleotide content. Phentolamine did not stimulate the postnatal accumulation of adenine nucleotides. In summary, the results show that the insulin-to-glucagon ratio is probably the most important hormone regulator of the rapid recompartmentation of adenine nucleotides into the mitochondrial matrix and that tissue oxygenation is strictly permissive for this hormone effect in the first 2 h after birth.