Dietary orotic acid, a new selective growth stimulus for carcinogen altered hepatocytes in rat.
Dietary orotic acid, a new selective growth stimulus for carcinogen altered hepatocytes in rat.
复制标题
膳食乳清酸是致癌物改变大鼠肝细胞的一种新的选择性生长刺激剂。
DOI：
10.1016/0304-3835(82)90060-x
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发表时间：
1982
期刊：
影响因子：
9.7
通讯作者：
Sarma,DS
中科院分区：
文献类型：
--
作者：
Columbano,A;Ledda,GM;Rao,PM;Rajalakshmi,S;Sarma,DS
观察到乳清酸（OA），嘧啶核苷酸生物合成的前体，当在饲料中以1%水平外源性供应时，选择性地刺激经1，2-二甲基肼（1，2-DMH）修饰的肝细胞的生长以形成γ-谷氨酰转移酶（γ-GT）（EC 2.3.2.2）阳性岛。将OA饮食的持续时间从5周增加到10周，导致病灶数量从6个/cm 2增加到14个/cm 2。然而，接受致癌物和基础饮食的大鼠仅产生1-2个病灶/cm 2。OA的这种独特作用可以通过提供肝细胞增殖刺激（例如单次坏死剂量的CCl 4）来进一步增强。
It was observed that orotic acid (OA), a precursor for pyrimidine nucleotide biosynthesis, when supplied exogenously at 1% level in the diet selectively stimulated the growth of hepatocytes modified by 1,2-dimethylhydrazine (1,2-DMH) to form γ-glutamyltransferase (γ-GT) (EC 2.3.2.2) positive islands. Increasing the duration of OA diet from 5 to 10 weeks resulted in an increase in the number of foci from 6 to 14/cm2. Rats that received the carcinogen and basal diet, however, developed only 1–2 foci/cm2. This unique effect of OA can be further accentuated by supplying a liver cell proliferative stimulus, such as a single necrogenic dose of CCl4.