Self-organization of histone-jointed three-dimensional DNA network
Self-organization of histone-jointed three-dimensional DNA network
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DOI：
10.1016/j.msec.2004.08.019
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发表时间：
2004-12
期刊：
影响因子：
--
通讯作者：
Y. Takeda;F. Mafuné;T. Kondow
中科院分区：
文献类型：
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作者：
Y. Takeda;F. Mafuné;T. Kondow
三维脱氧核糖核酸（DNA）网络在两个盖片之间的空间中自组织，其中充满了与多价阳离子（如Ca2+和组蛋白）混合的lambda DNA水溶液；前者作为覆盖滑移表面的DNA固定因子，后者作为网络的连接和支柱构建因子。用荧光显微镜观察了三维DNA网络的几何形状和组成部分。结果表明：(1)三维网络是基于在两个盖片内表面形成的二维网络，(2)三维网络由形成二维网络的结、连接两个相邻结的链和连接一个盖片表面上的二维网络的柱组成，(3)链由拉伸的λ DNA分子束组成。连接和柱子由dna -组蛋白复合物的聚集体构成。
A three-dimensional deoxyribonucleic acid (DNA) network was self-organized in a space between two cover slips, which was filled with an aqueous solution of lambda DNA mixed with multivalent cations such as Ca2+and histone; the former behaves as a DNA fixation factor on the cover-slip surface and the latter as a junction and pillar construction factor for the network. The geometry and the components of the three-dimensional DNA network thus formed were observed by fluorescence microscopy. It is revealed that (1) the three-dimensional network is based on two-dimensional networks formed on the inner surfaces of the two cover slips, (2) the three-dimensional network is composed of junctions for forming the two-dimensional networks, chains for connecting two adjacent junctions, and pillars connecting the two-dimensional network on one cover-slip surface to the other, and (3) the chains are made of bundles of stretched lambda DNA molecules, and the junctions and the pillars are made of the aggregates of DNA–histone complexes.