Interactions of radioprotectors and oxygen in cultured mammalian cells. II. Effects of dithiothreitol on radiation-induced DNA damage and comparison with cell survival.
Interactions of radioprotectors and oxygen in cultured mammalian cells. II. Effects of dithiothreitol on radiation-induced DNA damage and comparison with cell survival.
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培养的哺乳动物细胞中辐射防护剂和氧气的相互作用。
DOI：
10.2307/3576917
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发表时间：
1986
影响因子：
3.4
通讯作者：
D. Melder
中科院分区：
文献类型：
--
作者：
K. Held;G. Bren;D. Melder
用DNA解旋羟基磷灰石层析法和碱性滤膜洗脱法研究了含巯基化合物二硫苏糖醇（DTT）对辐射诱导的DNA损伤的影响。通过两种测定法测量的在空气中辐照的细胞的DNA损伤显示，在高DTT浓度下，药物浓度依赖性辐射防护达到高水平（剂量降低因子，DRF = 3）。针对DNA损伤的保护模式和程度与之前针对细胞存活所示的相同。然而，当细胞在缺氧条件下受到辐射时，通过解旋技术测量的DNA损伤在低DTT浓度下的减少程度低于存活率，但在高DTT浓度下DNA损伤的减少程度要大得多（DRF = 3）（与之相比）细胞存活的DRF = 1.5）。在所有浓度的DTT下，低氧照射后通过碱性洗脱测定法测量的DNA损伤在更大程度上降低，在1 mM时DRF = 1.6，在高水平的DTT下增加至DRF = 4.5。这些结果进行了讨论，在不同类型的DNA损伤中产生的细胞在空气中照射与缺氧和两种不同的DNA测定和细胞存活测量的损伤类型的差异。
The effects of the sulfhydryl-containing compound dithiothreitol (DTT) on radiation-induced DNA damage have been studied using two different assays: DNA unwinding hydroxyapatite chromatography and alkaline filter elution. DNA damage as measured by both assays for cells irradiated in air shows drug concentration-dependent radioprotection reaching high levels (dose reduction factor, DRF = 3) at high DTT concentrations. The pattern and degree of protection against DNA damage are the same as shown previously for cell survival. However, when cells are irradiated in hypoxia, DNA damage as measured by the unwinding technique is decreased less by low DTT concentrations than is survival, but DNA damage is decreased to a much greater extent (DRF = 3) at high concentrations of DTT (compared to DRF = 1.5 for cell survival). DNA damage as measured by the alkaline elution assay after hypoxic irradiation is decreased to a much greater extent at all concentrations of DTT with DRF = 1.6 at 1 mM and increasing to DRF = 4.5 at high levels of DTT. These results are discussed in terms of the different types of DNA damage produced in cells irradiated in air versus hypoxia and the differences in types of damage measured by the two different DNA assays and cell survival.
WR-2721 与放射治疗的临床试验。
DOI：
10.1016/0360-3016(82)90684-8
发表时间：
1982
期刊：
International journal of radiation oncology, biology, physics
影响因子：
--
作者：
Blumberg,AL;Nelson,DF;Gramkowski,M;Glover,D;Glick,JH;Yuhas,JM;Kligerman,MM
通讯作者：
Kligerman,MM
WR-2721联合烷化剂化疗的I期临床试验。
DOI：
10.1016/0360-3016(82)90687-3
发表时间：
1982
期刊：
International journal of radiation oncology, biology, physics
影响因子：
--
作者：
Glick,JH;Glover,DJ;Weiler,C;Blumberg,A;Nelson,D;Yuhas,JM;Kligerman,M
通讯作者：
Kligerman,M
哺乳动物细胞中脱氧核糖核酸链断裂及其修复的定量测定。
影响因子：
2.9
作者：
Blakely,WF;Ward,JF;Joner,EI
通讯作者：
Joner,EI