PREVENTION BY TIOPRONIN (2-MERCAPTOPROPIONYL GLYCINE) OF METHYLMERCURIC CHLORIDE-INDUCED TERATOGENIC AND FETO-TOXIC EFFECTS IN MICE
PREVENTION BY TIOPRONIN (2-MERCAPTOPROPIONYL GLYCINE) OF METHYLMERCURIC CHLORIDE-INDUCED TERATOGENIC AND FETO-TOXIC EFFECTS IN MICE
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DOI：
10.1002/tera.1420200213
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发表时间：
1979-01-01
期刊：
影响因子：
--
通讯作者：
HIRATA, S
中科院分区：
文献类型：
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作者：
FUJIMOTO, T;FUYUTA, M;HIRATA, S
以前的研究（Fuyuta等人，1976，1979）表明，在妊娠第10天对妊娠ICR小鼠单次经口给予25 mg/kg氯化甲基汞（MMC），可诱导胎仔腭裂的发生率非常高。阳性对照组20只母鼠于妊娠第10天经口给予25 mg/kg MMC，然后腹腔注射生理盐水。阴性对照组20只母鼠经口给予蒸馏水，然后腹腔注射生理盐水。阳性对照组98.1%的胎仔出现腭裂，阴性对照组无一例出现腭裂。20只雌性动物在妊娠第10天单次经口给予25 mg/kg MMC进行预处理，并经腹膜内给予硫普罗宁[Tp]进行后处理，MMC治疗后即刻及每24、48、72 h分别检测一次。TP的剂量为320、160和80 mg/kg/天。TP 320、160和80 mg/kg/d剂量组胎仔腭裂发生率分别降至1.49、31.3和47.8%。硫普罗宁能有效预防腭裂的发生。TP处理也有效预防了其他类型的异常以及以胎仔体重减轻为代表的胎儿毒性。
Previous investigations (Fuyuta et al., 1976, 1979) showed that a single oral administration of 25 mg/kg methylmercuric chloride (MMC) to pregnant ICR mice on day 10 of pregnancy induced cleft palate in a remarkably high incidence in fetuses. Twenty females in the positive control group were given 25 mg/kg MMC orally on day 10 of pregnancy and then given physiological saline i.p. Twenty females in the negative control group were given distilled H20 orally and then given saline i.p. Cleft palate was found in 98.1% of fetuses in the positive control group and none of them in the negative control group. Twenty females were pretreated with a single oral dose of 25 mg/kg MMC on day 10 of pregnancy and were posttreated with Tiopronin [Tp] i.p., immediately and at every 24, 48 and 72 h after the MMC treatment. The doses of Tp were 320, 160 and 80 mg/kg per day. The incidences of cleft palate in fetuses were reduced to 1.49, 31.3 and 47.8% in the Tp-treated groups with the doses of 320, 160 and 80 mg/kg per day, respectively. Tiopronin could effectively prevent the expected incidence of cleft palate. Other types of abnormalities as well as fetotoxicity represented by reduced fetal body weight were also effectively prevented with the Tp-treatment.