Elevated Serum MicroRNA Let-7c in Moyamoya Disease
Elevated Serum MicroRNA Let-7c in Moyamoya Disease
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烟雾病血清 MicroRNA Let-7c 升高
DOI：
10.1016/j.jstrokecerebrovasdis.2015.01.041
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发表时间：
2015
影响因子：
2.5
通讯作者：
Jia Yanjie
中科院分区：
文献类型：
--
作者：
Zhao Shaoyun;Gong Zhe;Zhang Jing;Xu Xiaoge;Liu Peidong;Guan Wenjuan;Jing Lijun;Peng Tao;Teng Junfang;Jia Yanjie
背景研究microRNA与烟雾病（moyamoya disease，MMD）的关系尚不多见。方法实验组49例MMD患者，30例正常人，20例脑出血患者，20例大面积脑梗死患者，20例非大面积脑梗死患者，20例神经系统自身免疫病患者作为对照组。Let-7家族水平通过聚合酶链反应测定。用双荧光素酶法检测let-7 c是否识别RNF 213的3′UTR。结果MMD患者let-7 c的表达水平明显高于对照组（P<0.001）。荧光素酶活性测定结果表明，hsa-let-7 c能显著降低含有RNF 213 3′-UTR的报告载体的荧光素酶活性（P< .05）。结论MMD患者let-7 c的表达增加可能通过靶向RNF 213而参与MMD的发病。因此，let-7 c可能成为诊断MMD的潜在生物标志物。
BackgroundFew studies have examined the relationship between mircroRNAs and moyamoya disease (MMD). We performed a study of the significance of let-7c expression in the serum of MMD patients.MethodsThe experimental group includes 49 MMD patients, and the control group consists of 30 normal people, 20 cerebral hemorrhage patients, 20 massive cerebral infarction patients, 20 nonmassive cerebral infarction patients, and 20 neurological autoimmune disease patients. Let-7 family levels were determined by polymerase chain reaction. A dual luciferase assay was used to test whether let-7c recognized the 3′UTR ofRNF213.ResultsThe expression level of let-7c in MMD patients is higher than that observed in the control groups (P< .001). The luciferase assay results indicated that hsa-let-7c could diminish luciferase activity from a reporter vector containing the 3′-UTR ofRNF213(P< .05). The suppression of luciferase activity is not found in mutRNF213(P> .05).ConclusionsIncreased expression of let-7c in MMD patients may contribute to MMD pathogenesis by targetingRNF213. Thus, let-7c may be a potential biomarker for the diagnosis of MMD.