Mineralization of gold nanoparticles using tailored M13 phages
Mineralization of gold nanoparticles using tailored M13 phages
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DOI：
10.1680/bbn.13.00004
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发表时间：
2013-12
期刊：
影响因子：
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通讯作者：
D. Rothenstein;S. Facey;M. Ploss;P. Hans;Melanie Melcher;V. Srot;P. Aken;B. Hauer;J. Bill
中科院分区：
文献类型：
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作者：
D. Rothenstein;S. Facey;M. Ploss;P. Hans;Melanie Melcher;V. Srot;P. Aken;B. Hauer;J. Bill
M13噬菌体外壳蛋白的基因工程为在纳米范围内无机材料的矿化提供了产生功能化生物模板的优良系统。这种生物模板为制造小型化器件开辟了简便的合成方法。金结合肽A3在M13颗粒表面上的两个不同位置表达，分别获得在一端或沿着整个噬菌体颗粒具有金结合肽的功能化生物模板。通过与胶体金颗粒的结合试验测试与各自的噬菌体外壳蛋白融合的金结合肽的功能性，并通过透射电子显微镜证实。与未修饰的M13噬菌体相比，工程化的噬菌体模板对金具有高亲和力。结合强度与M13噬菌体颗粒上表达的金结合肽的数量相关。从氯金酸溶液中矿化金纳米粒子（GNP）。T...
The genetic engineering of M13 phage coat proteins provides an excellent system for the generation of functionalized biotemplates for the mineralization of inorganic materials in the nanometer range. Such biotemplates open facile synthesis approaches for the fabrication of miniaturized devices. The gold-binding peptide A3 was expressed at two different locations on the M13 particle surface, obtaining functionalized biotemplates with either gold-binding peptides at the one end or along the whole phage particle, respectively. The functionality of the gold-binding peptides fused to the respective phage coat proteins was tested by a binding assay with colloidal gold particles and confirmed by transmission electron microscopy. The engineered phage templates have a high affinity to gold compared with non-modified M13 phages. The binding strength is correlated to the number of expressed gold-binding peptides on the M13 phage particles. Gold nanoparticles (GNPs) were mineralized from chloroauric acid solutions. T...