Enhancement of Cytoplasmic Maturation of In Vitro-Matured Pig Oocytes by Mechanical Vibration
Enhancement of Cytoplasmic Maturation of In Vitro-Matured Pig Oocytes by Mechanical Vibration
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DOI：
10.1262/jrd.09-142a
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发表时间：
2010-04-01
影响因子：
1.8
通讯作者：
Miyoshi, Kazuchika
中科院分区：
文献类型：
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作者：
Mizobe, Yamato;Yoshida, Mitsutoshi;Miyoshi, Kazuchika
研究了猪卵母细胞体外成熟和/或人工激活后体外培养过程中机械振动对成熟和发育的影响。并将优化的培养条件应用于小型猪体细胞核移植（SCNT）胚胎的体外培养。体外成熟过程中的机械振动不影响卵母细胞达到中期II期的比率（60.5 +/- 1.9-69.5 +/- 2.2%）。而机械振动间隔30-60 min 5s或间隔60 min 10 s激活成熟卵母细胞的囊胚形成率显著高于机械振动激活成熟卵母细胞（P
The effects of mechanical vibration during in vitro maturation and/or in vitro culture after artificial activation of pig oocytes on maturation and development were examined. In addition, the optimal conditions were applied to in vitro production of blastocysts derived from miniature pig somatic cell nuclear transfer (SCNT) embryos. Mechanical vibration during in vitro maturation did not affect the rates (60.5 +/- 1.9-69.5 +/- 2.2%) of oocytes reaching the metaphase-II stage. However, the blastocyst formation rates after activation of oocytes matured with mechanical vibration for 5 sec at intervals of 30-60 min or for 10 sec at intervals of 60 min were significantly (P