Immunoassay-aptasensor for the determination of tumor-derived exosomes based on the combination of magnetic nanoparticles and hybridization chain reaction.
Immunoassay-aptasensor for the determination of tumor-derived exosomes based on the combination of magnetic nanoparticles and hybridization chain reaction.
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基于磁性纳米颗粒和杂交链式反应相结合的免疫分析适体传感器测定肿瘤源性外泌体
DOI：
10.1039/d0ra10159a
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发表时间：
2021-01-25
期刊：
影响因子：
3.9
通讯作者：
Wu L
中科院分区：
文献类型：
--
作者：
Zhang H;Zhou Y;Luo D;Liu J;Yang E;Yang G;Feng G;Chen Q;Wu L
肿瘤相关外泌体的检测具有重要意义。在这项工作中，基于磁性纳米颗粒（MNPs）的捕获和对适体的特异性识别，设计了一种荧光适体传感器，用于检测肿瘤相关外泌体。利用MNPs作为底物，通过修饰MNP表面的CD63抗体来捕获外泌体。探针1由PDL-1适体序列和一段其他序列组成。外泌体表面表达PDL-1；PDL-1适配体能与PDL-1高亲和力结合。因此，形成了“mnps - exosome - probe 1”的免疫测定型化合物。探针1的另一段与探针2和探针3触发HCR，形成超长dsDNA。加入GelRed后，荧光信号被放大。该设计与外泌体浓度在300 ~ 107颗粒/ mL范围内具有良好的线性关系，检测限低至100颗粒/ mL。该配体传感器对肿瘤相关外泌体也具有高特异性，并成功应用于生物样品中。肿瘤相关外泌体的检测具有重要意义。
The detection of tumor-related exosomes is of great significance. In this work, a fluorescence aptasensor was designed for the determination of tumor-related exosomes based on the capture of magnetic nanoparticles (MNPs) and specific recognition of an aptamer. MNPs were used as substrates to capture the exosomes by modifying the CD63 antibody on the MNP surface. Probe 1 consists of PDL-1 aptamer sequence and a section of other sequences. PDL-1 expression was observed on the surface of exosomes; the aptamer of PDL-1 could combine with PDL-1 with high affinity. Thus, the immunoassay-type compounds of “MNPs–exosomes–probe 1” were formed. The other section of probe 1 triggered the HCR with probe 2 and probe 3 and formed the super-long dsDNA. The addition of GelRed resulted in the generation of an amplified fluorescence signal. The proposed design demonstrated a good linearity with the exosome concentration ranging from 300 to 107 particles per mL and with a low detection limit of 100 particles per mL. This aptasensor also exhibited high specificity for tumor-related exosomes, and was successfully applied in biological samples. The detection of tumor-related exosomes is of great significance.
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影响因子：
7.4
作者：
Sun, Yujiao;Jin, Hui;Gui, Rijun
通讯作者：
Gui, Rijun
影响因子：
2.7
作者：
Anjomshoa, Marzieh;Torkzadeh-Mahani, Masoud
通讯作者：
Torkzadeh-Mahani, Masoud
外泌体 PD-L1 有助于免疫抑制并与抗 PD-1 反应相关
影响因子：
64.8
作者：
Chen G;Huang AC;Zhang W;Zhang G;Wu M;Xu W;Yu Z;Yang J;Wang B;Sun H;Xia H;Man Q;Zhong W;Antelo LF;Wu B;Xiong X;Liu X;Guan L;Li T;Liu S;Yang R;Lu Y;Dong L;McGettigan S;Somasundaram R;Radhakrishnan R;Mills G;Lu Y;Kim J;Chen YH;Dong H;Zhao Y;Karakousis GC;Mitchell TC;Schuchter LM;Herlyn M;Wherry EJ;Xu X;Guo W
通讯作者：
Guo W
影响因子：
5.6
作者：
Kalimuthu, Kalishwaralal;Kwon, Woo Young;Park, Ki Soo
通讯作者：
Park, Ki Soo
DOI：
10.1007/s00249-014-0995-4
发表时间：
2015-02-01
影响因子：
2
作者：
Crisafuli, F. A. P.;Ramos, E. B.;Rocha, M. S.
通讯作者：
Rocha, M. S.