Effects of homocysteine thiol group on fibrin networks: another possible mechanism of harm
Effects of homocysteine thiol group on fibrin networks: another possible mechanism of harm
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DOI：
10.1016/s0049-3848(02)00191-3
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发表时间：
2002-07-15
影响因子：
7.5
通讯作者：
Kordich, LC
中科院分区：
文献类型：
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作者：
Lauricella, AM;Quintana, IL;Kordich, LC
根据越来越多的临床证据，众所周知，血浆同型半胱氨酸 (Hcy) 水平升高与静脉和动脉血栓形成的较高风险相关。为了阐明仍不清楚的机制，已经进行了几项实验研究。我们研究的目的是评估同型半胱氨酸对血浆纤维蛋白网络形成和结构的影响。我们还测定了同型半胱氨酸和半胱氨酸，以确定硫醇基团在测试活性中的可能参与。将与硫化合物单独孵育的血浆池的等分试样用凝血酶凝固。分别通过动力学研究和扫描电子显微镜评估纤维蛋白生成和纤维蛋白网络。与对照相比，所测定物质的纤维蛋白生成没有观察到显着差异。扫描电子显微镜显示，Hcy相关网络与对照不同，具有更短、更粗和更多分支的纤维，导致结构更紧凑，并且可能更能抵抗纤溶。硫醇基团将参与这种效应。我们的研究结果将为阐明与高同型半胱氨酸血症相关的有害影响的机制做出新的贡献。 (C) 2002 年，爱思唯尔科学有限公司出版。
On the basis of growing clinical evidence, it is well known that elevated plasma homocysteine (Hcy) levels are associated with higher risk of venous and arterial thrombosis. Several experimental studies have been carried out in order to elucidate the mechanisms involved that still remain unclear.The aim of our study was to evaluate the homocysteine effects on formation and structure of plasmatic fibrin network. We also assayed homocystine and cysteine to determinate possible participation of thiol group in the tested activity.Aliquots of a pool of plasma incubated separately with sulfur compounds were clotting with thrombin. Fibringeneration and fibrin networks were evaluated by kinetic studies and scanning electronic microscopy, respectively.No significant differences were observed on fibrin generation of the substances assayed in comparison to control. The scanning electronic microscopy showed that Hcy-associated networks were different from control, with shorter, thicker and more branched fibers, resulting in a more compact structure and probably more resistant to fibrinolysis. The thiol group would be involved in this effect. Our findings would be a new contribution to elucidate the mechanisms involved in harmful effects, associated to hyperhomocysteinemia. (C) 2002 Published by Elsevier Science Ltd.