PORCINE PARAROTAVIRUS - DETECTION, DIFFERENTIATION FROM ROTAVIRUS, AND PATHOGENESIS IN GNOTOBIOTIC PIGS
PORCINE PARAROTAVIRUS - DETECTION, DIFFERENTIATION FROM ROTAVIRUS, AND PATHOGENESIS IN GNOTOBIOTIC PIGS
复制标题
DOI：
10.1128/jcm.15.2.312-319.1982
复制
发表时间：
1982-01-01
影响因子：
9.4
通讯作者：
CROSS, RF
中科院分区：
文献类型：
--
作者：
BOHL, EH;SAIF, LJ;CROSS, RF
描述了一种新发现的猪肠道病毒的一些特性。然而，该病毒被称为猪副轮状病毒（PaRV），因为它在大小，形态和小肠绒毛肠细胞的嗜性方面类似于轮状病毒。它的抗原性不同于猪，人和牛轮状病毒和呼肠孤病毒1，2和3，和PaRV双链RNA的电泳迁移模式是不同于轮状病毒和呼肠孤病毒基因组的电泳迁移模式。通过在无菌猪中传代，从来自2个畜群的2头哺乳仔猪中分离到PaRV。在口服暴露于gnotobiotic猪后，小肠的绒毛肠细胞被感染，如通过免疫荧光判断的，导致绒毛萎缩和腹泻。当感染<5日龄的无菌猪时，死亡率高。该病毒C株在无菌猪体内连续传代10次，经电镜、免疫荧光和血清学试验均未发现外源性因子。病毒在培养基中含有胰酶的细胞培养物中连续传代5次，但复制可忽略不计或不存在，因为免疫荧光细胞的数量随着每次传代而减少。由于轮状病毒感染经常通过粪便标本的直接EM诊断，因此应考虑其他形态相似的病毒，如PaRV。建议使用免疫EM作为帮助识别这种情况的一种手段。
Some characteristics of a newly recognized porcine enteric virus are described. Tentatively, the virus was referred to as porcine pararotavirus (PaRV) because it resembled rotaviruses in respect to size, morphology and tropism for villous enterocytes of the small intestine. It was antigenically distinct from porcine, human and bovine rotaviruses and reoviruses 1, 2 and 3, and the electrophoretic migration pattern of PaRV double-stranded RNA was distinct from the electrophoretic migration patterns of the rotaviral and reoviral genomes. By passage in gnotobiotic pigs, PaRV was isolated from 2 suckling diarrheic pigs originating from 2 herds. After oral exposure of gnotobiotic pigs, villous enterocytes of the small intestines became infected as judged by immunofluorescence, resulting in villous atrophy and diarrhea. Mortality was high when gnotobiotic pigs < 5 days old were infected. The C strain of this virus was serially passed 10 times in gnotobiotic pigs and EM, immunofluorescence and serological tests indicated no extraneous agents. The virus was serially passed 5 times in cell cultures which contained pancreatin in the medium, but replication was negligible or absent, as the number of immunofluorescent cells decreased with each passage. Since rotaviral infections are frequently diagnosed by direct EM of fecal specimens, the presence of other morphologically similar viruses, such as PaRV, should be considered. The use of immune EM is suggested as a means of helping recognize this situation.