A low energy process for the recovery of bioproducts from cyanobacteria using a ball mill
A low energy process for the recovery of bioproducts from cyanobacteria using a ball mill
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DOI：
10.1016/j.bej.2012.08.010
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发表时间：
2012-12-15
影响因子：
3.9
通讯作者：
Llewellyn, Carole A.
中科院分区：
文献类型：
--
作者：
Balasundaram, Bangaru;Skill, Stephen C.;Llewellyn, Carole A.
高效、经济的微藻大规模生物处理生产一系列生物制品。朝着生物精炼的方向努力，对藻类工业生物技术的成功至关重要。使用高通量细胞破坏系统和蓝藻作为模式生物，研究了影响不同亚细胞位置产品回收的关键过程变量。这些信息随后被用于设计和测试差别化回收程序的中试规模，使用定制设计的球磨机，消耗1.87千瓦时/公斤干生物质，比报道的最佳藻类破坏系统低约34%。通过控制磨矿介质与微生物之间碰撞次数和每次碰撞力的平衡，实现差别化回收。采用高效节能的固液分离技术——重力沉降法，在第一步和第二步球磨结束时，固相回收率分别大于99%和95%。基于这些结果和细胞内和/或细胞外产物的释放率测试，提出了一个理论框架，为设计差别化回收过程使用球磨机。(C) 2012 Elsevier B.V.版权所有
Efficient and economical large scale bioprocessing of microalgae to produce a range of bio-products. working towards a biorefinery approach, is critical for the success of algal industrial biotechnology. The key process variables that affect the recovery of products from different sub-cellular locations were investigated using a high throughput cell disruption system and a cyanobacterium as model organism. This information was then used to design and test a differential recovery procedure at pilot scale using a custom designed ball mill that consumed 1.87 kWh/kg of dry biomass used approximately 34% lower than the best algal disruption system reported. The balance between the number of collisions and force of each collision between grinding media and the microorganism were manipulated to achieve differential recovery. Greater than 99 and 95% solids were recovered at the end of first and second ball milling step respectively through gravity sedimentation, an energy efficient solid-liquid separation technique. Based on these results and the release rates of intracellular and/or extracellular products tested, a theoretical framework is presented for the design of a differential recovery process using ball mills. (C) 2012 Elsevier B.V. All rights reserved.