The molecular effects of alcohol: clues to the enigmatic action of alcohol.
The molecular effects of alcohol: clues to the enigmatic action of alcohol.
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酒精的分子效应：酒精神秘作用的线索。
DOI：
10.1111/j.1749-6632.2003.tb07515.x
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发表时间：
2003
影响因子：
5.2
通讯作者：
Ticku,MaharajK
中科院分区：
文献类型：
--
作者：
Kumari,Meena;Anji,Antje;WoodsJr,Henri;Ticku,MaharajK
摘要：慢性乙醇处理(50 mM，5天)可稳定培养的胎脑皮质神经元NR1受体亚单位mRNA。本文探讨了乙醇调节NR1mRNA表达的机制(S)。具体地说，我们已经确定了NR1mRNA在皮质神经元中的细胞定位和/或新蛋白的合成是否是乙醇介导的NR1mRNA稳定所必需的。亚细胞分级研究表明，所有可检测到的NR1mRNA都与粗面内质网有关，这表明NR1mRNA在胚胎皮质神经元中的亚细胞分布在乙醇介导的NR1mRNA稳定中没有发挥作用。但放线菌酮对蛋白质合成的抑制可阻断乙醇对NR1mRNA的稳定作用，提示新蛋白的合成是乙醇稳定NR1mRNA的关键。
Abstract:Chronic ethanol treatment (50 mM, five days) induces stabilization of NR1 receptor subunit mRNA in cultured fetal cortical neurons. In this paper, we investigate the mechanism(s) by which ethanol mediates its effects on NR1 mRNA. Specifically, we have determined if cellular localization of NR1 mRNA in cortical neurons and/orde novoprotein synthesis is essential for ethanol‐mediated stabilization of NR1 mRNA. Subcellular fractionation studies show that all detectable NR1 mRNA is associated with rough endoplasmic reticulum, indicating that subcellular distribution of NR1 mRNA in fetal cortical neurons does not play a role in ethanol‐mediated NR1 mRNA stabilization. However, inhibition of protein synthesis by cycloheximide abolished the mRNA stabilizing effect of ethanol on NR1 mRNA, thus suggestingde novoprotein synthesis is crucial for the action of ethanol on NR1 mRNA stabilization.