Heterogeneity of nitric oxide synthase-containing neurons in the mouse main olfactory bulb
Heterogeneity of nitric oxide synthase-containing neurons in the mouse main olfactory bulb
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DOI：
10.1016/j.neures.2006.10.005
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发表时间：
2007-02-01
影响因子：
2.9
通讯作者：
Kosaka, Katsuko
中科院分区：
文献类型：
--
作者：
Kosaka, Toshio;Kosaka, Katsuko
研究了小鼠主嗅球内含一氧化氮合酶（NOS）神经元的分布和结构特征。NOS阳性神经元呈异质性，包括肾小球周围细胞、颗粒细胞、外丛状层中间神经元、浅层和深层短轴突细胞及星状细胞等。NOS阳性肾小球周围细胞常呈钙结合蛋白免疫反应阳性，但很少呈钙结合蛋白阳性。重要的是，一些中间和外部的簇状细胞也被证实是NOS阳性，其中一些也是胆囊收缩素（CCK）阳性。逆行示踪实验表明，部分NOS阳性簇状细胞和CCK阳性簇状细胞构成了嗅球内联合系统和向嗅结节的投射系统。此外，另一个特定的NOS阳性神经元的亚群，没有或很少CCK免疫反应性似乎投射到覆盖的背核，屏状核和岛皮质的区域。此外，不同类型的神经元以外的二尖瓣/簇状细胞也被认为是投射神经元的MOB。本研究揭示了小鼠MOB中NOS阳性神经元的多样性，并进一步揭示了它们在结构和化学性质上与以前报道的大鼠MOB中的NOS阳性神经元不同。(c)2006年Elsevier爱尔兰有限公司和日本神经科学学会。All rights reserved.
The distribution and structural features of nitric acid synthase (NOS) containing intrinsic neurons were studied in the mouse main olfactory bulb (MOB). NOS positive neurons were heterogeneous, including some subpopulations of periglomerular cells, granule cells, intemeurons in the external plexiform layer, superficial and deep short-axon cells and stellate cells. NOS positive periglomerular cells were frequently calretinin immunoreactive and, although rarely, calbindin positive. Importantly, some middle and external tufted cells were also confirmed to be NOS positive, some of which were also cholecystokinin (CCK) positive. Retrograde tracer experiments showed that some NOS positive tufted cells, which were also CCK positive, constitute the intrabulbar association system and the projection system to the olfactory tubercle. In addition, another particular subpopulation of NOS positive neurons with no or little CCK immunoreactivity appeared to project to areas covering the dorsal endopiriform nucleus, claustrum and insular cortex. Furthermore, diverse types of neurons other than mitral/tufted cells were also suggested to be projection neurons of the MOB. The present study revealed the diversity of NOS positive neurons in the mouse MOB and further revealed that they were different from those reported previously in the rat MOB in structural and chemical properties. (c) 2006 Elsevier Ireland Ltd and the Japan Neuroscience Society. All rights reserved.