Effects of Multiple Injection of Bone Marrow Mononuclear Cells on Spinal Cord Injury of Rats
Effects of Multiple Injection of Bone Marrow Mononuclear Cells on Spinal Cord Injury of Rats
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DOI：
10.1089/neu.2016.4841
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发表时间：
2017-11-01
影响因子：
4.2
通讯作者：
Ide, Chizuka
中科院分区：
文献类型：
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作者：
Kanekiyo, Kenji;Nakano, Norihiko;Ide, Chizuka
比较多次注射骨髓单个核细胞（BMNCs）与单次注射对大鼠脊髓损伤（SCI）的影响。通过密度梯度离心从骨髓灌注液中分离的BMNCs通过第四脑室通过脑脊液（CSF）注射三次（每周一次），并将运动改善和组织恢复（包括轴突再生）与单次注射进行比较。单次注射组在移植后1周表现出Basso-Beattie-Bresnahan（BBB）评分的急剧升高，而多次注射组在移植后2周保持类似的急剧升高，并且多次注射组的BBB评分此后保持在比单次注射组高约2-3分的水平，直到实验结束。在移植后3、4和8周，单次和多次注射组之间存在显著差异。移植后8周BBB评分的差异表明，在此阶段单次注射组和多次注射组之间的运动行为质量存在显著差异。在单次和多次注射组中，发现再生轴突通过星形胶质细胞缺失区域广泛生长，空腔形成明显减少。然而，在单次和多次注射组之间，再生轴突的密度或空腔的体积没有显著差异。这些结果表明，尽管单次和多次注射之间的组织恢复相似，但多次注射BMNC比单次注射治疗SCI更有利于运动改善。由于BMNCs的制备和注射技术简单、成本低，腰椎穿刺多次注射BMNCs比单次注射具有更大的临床应用优势。
The effects of multiple injection of bone marrow mononuclear cells (BMNCs) on spinal cord injury (SCI) were compared with those of single injection in rats. BMNCs separated by density-gradient centrifugation from a bone marrow perfusate were injected three times (once weekly) through the cerebrospinal fluid (CSF) via the fourth ventricle, and the locomotor improvement and tissue recovery, including axonal regeneration, were compared with those of single injection. While the single-injection group showed a steep elevation of the Basso-Beattie-Bresnahan (BBB) score 1 week after transplantation, the multiple-injection group maintained a similar steep elevation for 2weeks after transplantation, and the BBB scores of the multiple-injection group remained thereafter at a level approximately 2-3 points higher than those of the single-injection group until the end of the experiment. There were significant differences between the single- and multiple-injection groups at 3, 4, and 8 weeks after transplantation. The difference in BBB scores at 8 weeks after transplantation suggested that there was a marked difference in the quality of locomotor behaviors between the single-and multiple-injection groups at this stage. An extensive outgrowth of regenerating axons through the astrocyte-devoid areas and a marked reduction of cavity formation were found in both the single-and multiple-injection groups. There were, however, no significant differences in the density of regenerating axons or volumes of cavities between the single-and multiple-injection groups. These results showed that although tissue recoveries were similar between single and multiple injection, the multiple injection of BMNCs was more beneficial for locomotor improvement than single injection for the treatment of SCI. Considering the technically simple and low-cost procedures for the preparation and injection of BMNCs, multiple injection of BMNCs by lumbar puncture has an advantage over single injection on clinical application.