Vasopressin and oxytocin receptor mRNA expression during rat telencephalon development
Vasopressin and oxytocin receptor mRNA expression during rat telencephalon development
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DOI：
10.1054/npep.2000.0809
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发表时间：
2000-06-01
期刊：
影响因子：
2.9
通讯作者：
Brinton, RD
中科院分区：
文献类型：
--
作者：
Chen, Q;Schreiber, SS;Brinton, RD
我们使用半定量逆转录聚合酶链反应 (RT-PCR) 和 Southern 印迹杂交研究了加压素和催产素受体以及肽 mRNA 的发育表达。从胚胎第 12 天到第 20 天，端脑中存在加压素受体亚型 V(1)a 和 V-2 的信使 RNA。V(1)a 和 V-2 受体 mRNA 均在第 13 天增加，然后从胚胎第 13 天到第 20 天保持稳定。从第 12 天到第 20 天，在端脑中也检测到加压素肽的信使 RNA，表明在大鼠胚胎发育过程中，加压素可在大鼠大脑皮层内合成。催产素受体 mRNA 表达也存在于端脑中，但表达水平从第 12 天到第 20 天变化很大。在大鼠端脑发育过程中未检测到催产素 mRNA 表达。加压素受体和加压素肽 mRNA 表达以及催产素受体 mRNA 的时间模式表明，加压素和催产素介导的作用在大鼠端脑发育过程中具有时间作用。 (C) 2000 哈考特出版有限公司
We investigated the developmental expression of vasopressin and oxytocin receptor and peptide mRNA using semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) and Southern blot hybridization. Messenger RNAs for both vasopressin receptor subtypes V(1)a and V-2 were present in the telencephalon from embryonic day 12 to day 20. Both V(1)a and V-2 receptor mRNA increased on day 13 and then remained stable from embryonic day 13 to day 20. Messenger RNA for the vasopressin peptide was also detected in the telencephalon from day 12 to day 20, indicating that vasopressin could be synthesized within the rat cerebral cortex during rat embryonic development. Oxytocin receptor mRNA expression was also present in the telencephalon, but expression levels varied considerably from day 12 to day 20. No oxytocin mRNA expression was detected during rat telencephalon development. Temporal patterns of vasopressin receptor and vasopressin peptide mRNA expression along with oxytocin receptor mRNA suggest a temporal role for vasopressin- and oxytocin-mediated actions during rat telencephalon development. (C) 2000 Harcourt Publishers Ltd.