The effect of solution nonideality on modeling transmembrane water transport and diffusion-limited intracellular ice formation during cryopreservation
The effect of solution nonideality on modeling transmembrane water transport and diffusion-limited intracellular ice formation during cryopreservation
复制标题
溶液非理想性对冷冻保存期间跨膜水运输和扩散限制细胞内冰形成建模的影响
DOI：
10.1063/1.4870826
复制
发表时间：
2014-04-14
影响因子：
3.2
通讯作者：
He, Xiaoming
中科院分区：
文献类型：
--
作者：
Zhao, Gang;Takamatsu, Hiroshi;He, Xiaoming
开发了一种新的模型来预测跨膜水传输和扩散限制的冰形成在细胞冷冻过程中没有理想的解决方案的假设，已在以前的模型。该模型被应用于预测细胞脱水和细胞内冰形成（IIF）的小鼠卵母细胞和牛颈动脉内皮细胞在氯化钠（NaCl）水溶液中与甘油作为冷冻保护剂或冷冻保护剂的冷冻保存过程中。本模型与先前报道的模型之间的预测比较表明，理想溶液假设导致在缓慢冷却速率下细胞内冰的量的预测不足（
A new model was developed to predict transmembrane water transport and diffusion-limited ice formation in cells during freezing without the ideal-solution assumption that has been used in previous models. The model was applied to predict cell dehydration and intracellular ice formation (IIF) during cryopreservation of mouse oocytes and bovine carotid artery endothelial cells in aqueous sodium chloride (NaCl) solution with glycerol as the cryoprotectant or cryoprotective agent. A comparison of the predictions between the present model and the previously reported models indicated that the ideal-solution assumption results in under-prediction of the amount of intracellular ice at slow cooling rates (