Cell-autonomous requirements for Dlg-1 for lens epithelial cell structure and fiber cell morphogenesis.
Cell-autonomous requirements for Dlg-1 for lens epithelial cell structure and fiber cell morphogenesis.
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DOI：
10.1002/dvdy.22036
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发表时间：
2009-09
影响因子：
2.5
通讯作者：
Griep, Anne E.
中科院分区：
文献类型：
--
作者：
Rivera, Charlene;Yamben, Idella F.;Shatadal, Shalini;Waldof, Malinda;Robinson, Michael L.;Griep, Anne E.
细胞的极性和粘附性被认为是生物体发育的关键决定因素。在果蝇体内，大圆盘(DLG)已成为上皮细胞增殖、黏附和极性的重要调节因子。在此，我们研究了小鼠DLG同源基因(DLG-1)在小鼠晶状体发育中的作用。在晶状体发育早期，对整个晶状体进行组织特异性的DLG-1消融，会导致晶状体上皮细胞的扩张和紊乱，这与黏附因子和极性因子的分布变化有关。在纤维细胞中观察到分化缺陷。这些变化包括细胞结构的改变和细胞黏附/细胞骨架因子的处置，去核延迟，以及α-连环蛋白、pERK1/2和MIP26水平的降低。当DLG-1仅在纤维细胞中被破坏时，这些纤维细胞缺陷被重现。这些结果表明，DLG-1以细胞自主的方式调节上皮细胞结构和纤维细胞分化。
Cell polarity and adhesion are thought to be key determinants in organismal development. In Drosophila, discs large (dlg) has emerged as an important regulator of epithelial cell proliferation, adhesion and polarity. Herein, we investigated the role of the mouse homolog of dlg (Dlg-1) in the development of the mouse ocular lens. Tissue specific ablation of Dlg-1 throughout the lens early in lens development led to an expansion and disorganization of the epithelium that correlated with changes in the distribution of adhesion and polarity factors. In the fiber cells differentiation defects were observed. These included alterations in cell structure and the disposition of cell adhesion/cytoskeletal factors, delay in denucleation, and reduced levels of α-catenin, pERK1/2 and MIP26. These fiber cell defects were recapitulated when Dlg-1 was disrupted only in fiber cells. These results suggest that Dlg-1 acts in a cell autonomous manner to regulate epithelial cell structure and fiber cell differentiation.
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影响因子：
64.5
作者：
Drees, F;Pokutta, S;Weis, WI
通讯作者：
Weis, WI
影响因子：
2.7
作者：
Chen, Yongjuan;Stump, Richard J. W.;Lovicu, Frank J.;Shimono, Akihiko;McAvoy, John W.
通讯作者：
McAvoy, John W.
DOI：
10.1073/pnas.94.21.11612
发表时间：
1997-10-14
影响因子：
11.1
作者：
Kiyono, T;Hiraiwa, A;Ishibashi, M
通讯作者：
Ishibashi, M
影响因子：
2.9
作者：
Iyengar, Laxmi;Wang, Qian;Lovicu, Frank J.
通讯作者：
Lovicu, Frank J.
影响因子：
3.3
作者：
Firestein, BL;Rongo, C
通讯作者：
Rongo, C