Host and tissue specificity of Trichomonas vaginalis is not mediated by its known adhesion proteins
Host and tissue specificity of Trichomonas vaginalis is not mediated by its known adhesion proteins
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DOI：
10.1128/iai.68.7.4358-4360.2000
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发表时间：
2000-07-01
影响因子：
3.1
通讯作者：
Fiori, PL
中科院分区：
文献类型：
--
作者：
Addis, MF;Rappelli, P;Fiori, PL
阴道毛滴虫的粘附被认为依赖于四种粘附蛋白，认为它们以配体-受体类型的相互作用以特异性方式与阴道上皮细胞结合。然而，宿主细胞上的特异性受体尚未被鉴定。在这项工作中，T.已经研究了迷走神经粘附素与不同组织学来源和来自不同物种的细胞的结合。HeLa、CHO和Vero细胞系;来自不同物种的红细胞;以及无细胞壁的原核生物人型支原体，用于研究T.迷走神经粘附素我们观察到T.迷走神经粘附素能够以相同的程度结合不同的细胞类型，这表明T.迷走神经粘附不应是由于寄生虫粘附素的特异性。我们的研究结果表明，迄今为止发表的关于这个问题的数据可能是人为的，在文献中报道的实验是不适合识别原生动物粘附素。
Adhesion of Trichomonas vaginalis is believed to be dependent on four adhesion proteins, which are thought to bind to vaginal epithelial cells in a specific manner with a ligand-receptor type of interaction. However, the specific receptors on the host cell have not yet been identified. In this work, the ability of the T. vaginalis adhesins to bind to cells of different histologic derivations and from different species has been studied. HeLa, CHO, and Vero cell lines; erythrocytes from different species; and a prokaryote without a cell wall, Mycoplasma hominis, were employed in order to investigate the cell specificity of the T. vaginalis adhesins. We observed that the T. vaginalis adhesins are able to bind to the different cell types to the same extent, suggesting; that the host and tissue specificity of T. vaginalis adhesion should not be due to specificity of the parasite adhesins. Our results suggest that the data published to date on the subject are probably artifactual and that the experiments reported in the literature are not appropriate for identification of protozoan adhesins.