Response of Ig-positive cells to Enteromyxum scophthalmi (Myxozoa) experimental infection in turbot, Scophthalmus maximus (L.):: A histopathological and immunohistochemical study
Response of Ig-positive cells to Enteromyxum scophthalmi (Myxozoa) experimental infection in turbot, Scophthalmus maximus (L.):: A histopathological and immunohistochemical study
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DOI：
10.1016/j.fsi.2006.02.006
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发表时间：
2006-11-01
影响因子：
4.7
通讯作者：
Nieto, J. M.
中科院分区：
文献类型：
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作者：
Bermudez, R.;Vigliano, F.;Nieto, J. M.
近年来，在西班牙西北部的养殖大比目鱼中发现了一种新的寄生虫，造成了严重的损失。死鱼表现出消瘦和恶病质，这是由严重的坏死性肠炎引起的，影响到消化道的所有部分。该寄生虫被归类为粘孢子虫，并命名为scphthahni肠粘菌。这项研究是为了深入了解鱼类对这种寄生虫感染的免疫反应。研究了实验感染华支睾吸虫的大比目鱼脾、肾、肠免疫球蛋白阳性(Ig+)细胞的动态变化。为了评估感染的进展和寄生虫引起的损害，我们进行了组织病理学研究，并使用间接免疫组织化学方法原位检测Ig+细胞。在接触寄生虫的鱼中，其靶器官肠道的Ig+细胞数量显著增加，而在未检测到寄生虫的脾和肾脏的造血器官中，Ig+细胞的数量减少。此外，与对照鱼(未接触寄生虫)相比，受体/受感染鱼的所有三个器官中Ig+细胞的分布模式发生了变化。本研究结果表明，大菱鲆感染棘球绦虫可引起免疫应答，免疫应答涉及免疫球蛋白阳性细胞的数量和分布的变化。(C)2006爱思唯尔有限公司。保留所有权利。
In recent years, a new parasite that causes severe losses has been detected in farmed turbot, Scophthalmus maximus (L.), in Northwestern Spain. Dead fish showed emaciation and cachexia caused by severe necrotizing enteritis that affected all portions of the digestive tract. The parasite was classified as a myxosporean and named Enteromyxum scophthahni. This study was set up to gain insights into the immune response of fish against this parasitic infection. The kinetics of immunoglobulin positive (Ig+) cells in spleen, kidney and intestine from turbot experimentally infected with E. scophthalmi was studied. For evaluating both the progress of infection and the lesions induced by the parasite, we performed histopathological studies and for detecting Ig+ cells in situ we used an indirect immunohistochemical method. In fish exposed to the parasite, the number of Ig+ cells significantly increased in the intestine, the target organ of the parasite, whereas in spleen and kidney, haematopoietic organs where the parasite was not detected, the number of Ig+ cells decreased. Furthermore, the pattern of distribution of Ig+ cells changed in all three organs examined in recipient/infected fish compared with control fish (not exposed to the parasite). The results obtained in this study indicate that the infection by E. scophthalmi in turbot induced an immune response that involved changes in the number and distribution of Ig+ cells. (c) 2006 Elsevier Ltd. All rights reserved.