Urinary oxidative stress markers in young patients with type 1 diabetes
Urinary oxidative stress markers in young patients with type 1 diabetes
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DOI：
10.1111/j.1442-200x.2006.02156.x
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发表时间：
2006-02-01
影响因子：
1.4
通讯作者：
Mayumi, M
中科院分区：
文献类型：
--
作者：
Hata, I;Kaji, M;Mayumi, M
背景：氧化应激参与了糖尿病血管并发症的发病机制。然而，有几种方法来确定氧化应激的状态，直接和定量的年轻患者1型diabetes.Methods：共27例年轻1型糖尿病患者（平均年龄+/- SD，12.6 +/- 4.2岁）与肾功能正常和38名健康对照组（13.0 +/- 4.6岁）进行了调查。收集清晨排尿尿液样本。采用竞争性酶联免疫吸附法（ELISA）测定尿中丙烯醛-赖氨酸加合物、8-羟基-2 '-脱氧鸟苷（8-OHdG）的浓度，采用比色法、非酶法测定尿中一氧化氮代谢产物的浓度。对于糖尿病患者，微量白蛋白尿与所有三种标志物的较高浓度显著相关。血红蛋白A（1c）值与8-OHdG values.Conclusion显着相关：这些研究结果表明，增加氧化应激和血管并发症的风险可能存在于1型糖尿病的早期阶段。
Background: Involvement of oxidative stress in the pathogenesis of diabetic vascular complications has been proposed. However, there are few methods to determine the status of oxidative stress both directly and quantitatively in young patients with type 1 diabetes.Methods: A total of 27 young patients with type 1 diabetes (mean age +/- SD, 12.6 +/- 4.2 years) with normal renal function and 38 healthy control subjects (13.0 +/- 4.6 years) were investigated. Early morning voiding urine samples were collected. The concentrations of acrolein-lysine adducts, 8-hydroxy-2'-deoxyguanosine (8-OHdG) were determined using competitive enzyme-linked immunosorbent assay, and nitric oxide metabolites were measured using the colorimetric, non-enzymatic assay.Results: Urinary concentrations of 8-OHdG, but not acrolein-lysine adducts and nitric oxide metabolites, were significantly increased in the diabetic group. For diabetic patients, microalbuminuria was significantly correlated with higher concentrations of all three markers. Hemoglobin A(1c) values were significantly correlated with 8-OHdG values.Conclusion: These findings indicate that increased oxidative stress and the risk of vascular complications may be present at early stages of type 1 diabetes.