Phospholamban modulates murine atrial contractile parameters and responses to β-adrenergic agonists
Phospholamban modulates murine atrial contractile parameters and responses to β-adrenergic agonists
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DOI：
10.1006/jmcc.1998.0688
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发表时间：
1998-07-01
影响因子：
5
通讯作者：
Kranias, EG
中科院分区：
文献类型：
--
作者：
Kadambi, VJ;Koss, KL;Kranias, EG
与鼠心室相比，鼠心房中受磷蛋白基因转录物水平低得多，并且已经表明这种受磷蛋白表达的降低导致增强的心房收缩参数。为了描述受磷蛋白在鼠心房中的功能作用，评价了来自受磷蛋白敲除小鼠和心脏特异性受磷蛋白过表达小鼠沿着它们的同基因野生型对照的分离肌肉的收缩参数。评估起搏心房肌的张力发展和半松弛的时限（ms），以及张力发展和松弛的速率（mg/s），显示受磷蛋白消融与松弛速率增强相关，对收缩速率无显著影响。受磷蛋白过表达（三倍）与收缩和舒张速率的降低有关，而异丙肾上腺素刺激导致受磷蛋白缺乏和受磷蛋白过表达的心房中产生的张力和舒张速率的显著增加，表明β-肾上腺素能途径在这些肌肉中起作用。这些发现表明受磷蛋白是心房收缩力及其对β-肾上腺素能激动剂反应的重要调节剂。(C)北京：科学出版社.
Phospholamban gene transcript levels are much lower in murine atria as compared to murine ventricles and this reduced phospholamban expression has been suggested io result in enhanced atrial contractile parameters, To delineate the functional role of phospholamban in murine atrium, the contractile parameters of isolated muscles from phospholamban knockout and cardiac-specific phospholamban overexpression mice along with their isogenic wild-type controls were evaluated, Assessment of the limes (ms) to peal; tension development and to half-relaxation of developed tension, as well as the rates (mg/s) of tension development and relaxation in paced atrial muscles, revealed that phospholamban ablation was associated with enhanced rates of relaxation with no significant effect on contraction rate. while phospholamban overexpression (three-fold) was associated with depressed rates of both contraction and relaxation, Isoproterenol stimulation resulted in significant increases in the rates of developed tension and relaxation in both phospholamban deficient and phospholamban overexpression atria, indicating that the beta-adrenergic pathway was functional in these muscles. These findings suggest that phospholamban is an important modulator of atrial contractility and its responses to beta-adrenergic agonists. (C) 1998 Academic Press.