Short-term ethanol exposure alters calbindin D28k and glial fibrillary acidic protein immunoreactivity in hippocampus of mice
Short-term ethanol exposure alters calbindin D28k and glial fibrillary acidic protein immunoreactivity in hippocampus of mice
复制标题
短期乙醇暴露改变小鼠海马钙结合蛋白 D28k 和胶质纤维酸性蛋白免疫反应性
DOI：
10.1016/s0006-8993(00)02729-3
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发表时间：
2000
期刊：
影响因子：
2.9
通讯作者：
Y. Takeuchi
中科院分区：
文献类型：
--
作者：
I. Satriotomo;T. Miki;M. Itoh;K. Ameno;I. Ijiri;Y. Takeuchi
The effects of a short-term ethanol treatment on hippocampus have been studied in mice exhibiting intoxication signs. The alterations of neurons and astrocytes as well as quantitative changes of calbindin D28k-immunoreactivity and glial fibrillary acidic protein-immunoreactivity (GFAP-IR) in selected regions of the dorsal hippocampus were examined using anti-calbindin and anti-GFAP monoclonal anti-body (mAb), respectively. The administration of 6% (v/v) ethanol during first week led to the neuronal death and decrease of the total number of calbindin-IR neurons in the examined brain regions. Moreover, the calbindin positive neurons were shown to have diminished processes following short-term ethanol exposure. These neuronal changes were associated with the increase of the GFAP-IR astrocytes. Hypertrophy of cell bodies and cytoplasmic processes of reactive astrocytes were also seen. In addition, dense, thick and highly-stained GFAP-IR cells with long processes in granular cell layer appeared entering into molecular layer of dentate gyrus. In agreement with the discrepancy percentage of neuronal cell loss and increase of reactive astrocytes detected by calbindin and GFAP-IR using image quantitative analysis, the regional differences in the vulnerability to the neurotoxic effects following short-term ethanol exposure were found: CA3>CA2>CA1>DG. These findings also illustrate the importance of correlation between calbindin and GFAP-IR when determining the morphological alteration of neuron and astroglial following short-term ethanol treatment. The disruption of calbindin and GFAP could affect neuronal-astroglial interaction, resulting in disturbance of behaviors dependent on hippocampus.
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DOI：
10.1080/15287399309531721
发表时间：
1993-03
期刊：
Journal of toxicology and environmental health
影响因子：
--
作者：
M. Aschner;R. LoPachin
通讯作者：
M. Aschner;R. LoPachin
影响因子：
2.3
作者：
D. Pierce;J. West
通讯作者：
D. Pierce;J. West
DOI：
10.1111/j.1530-0277.1990.tb00455.x
发表时间：
1990-02-01
期刊：
ALCOHOL-CLINICAL AND EXPERIMENTAL RESEARCH
影响因子：
--
作者：
BONTHIUS, DJ;WEST, JR
通讯作者：
WEST, JR
胎儿酒精暴露：细胞毒性和毒性涉及的分子事件。
DOI：
10.1111/j.1530-0277.1990.tb01821.x
发表时间：
1990
期刊：
Alcoholism, clinical and experimental research
影响因子：
--
作者：
Michaelis,EK
通讯作者：
Michaelis,EK
有毒物质增加细胞内游离 Ca2：潜在神经毒性的指标？
影响因子：
13.8
作者：
Komulainen,H;Bondy,SC
通讯作者：
Bondy,SC