Comparative Analysis of Microcystin Prevalence in Michigan Lakes by Online Concentration LC/MS/MS and ELISA
Comparative Analysis of Microcystin Prevalence in Michigan Lakes by Online Concentration LC/MS/MS and ELISA
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DOI：
10.3390/toxins11010013
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发表时间：
2019-01-01
期刊：
影响因子：
4.2
通讯作者：
Szlag, David C.
中科院分区：
文献类型：
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作者：
Birbeck, Johnna A.;Westrick, Judy A.;Szlag, David C.
需要快速可靠的工作流程来定量微囊藻毒素（MC），这是一种普遍存在的肝毒性蓝藻毒素，以便快速评估人类和环境暴露的影响并将其降至最低。我们的目标是开发一种高通量在线浓缩液相色谱串联质谱（LC/MS/MS）工作流程，以定量地表水和饮用沃茨中的12种市售MCs和节球藻素。该方法的运行时间为8.5分钟，检测限在低ng/L范围内，最低报告水平在5至10 ng/L之间。通过确定MC的流行率并将Adda-ELISA定量与我们来自密歇根州31个水体的122个样本的新工作流程进行比较，对该工作流程进行了基准测试。MC出现频率为MC-LA > LR > RR > D-Asp（3）-LR > YR > HilR > WR > D-Asp（3）-RR > HtyR > LY = LW = LF，其中MC-RR浓度最高。在33个样品中检测到MC，其中13个样品的MC总浓度超过20%，而美国环境保护署（US EPA）方法544中不存在MC。此外，LC/MS/MS和Adda-ELISA数据之间的季节性偏差表明Adda-ELISA与MC降解产物发生交叉反应。该工作流程提供了少于24小时的定量周转时间，并确定了LC/MS/MS和ELISA定量之间的关键差异，应进一步研究。
Fast and reliable workflows are needed to quantitate microcystins (MCs), a ubiquitous class of hepatotoxic cyanotoxins, so that the impact of human and environmental exposure is assessed quickly and minimized. Our goal was to develop a high-throughput online concentration liquid chromatography tandem mass spectrometry (LC/MS/MS) workflow to quantitate the 12 commercially available MCs and nodularin in surface and drinking waters. The method run time was 8.5 min with detection limits in the low ng/L range and minimum reporting levels between 5 and 10 ng/L. This workflow was benchmarked by determining the prevalence of MCs and comparing the Adda-ELISA quantitation to our new workflow from 122 samples representing 31 waterbodies throughout Michigan. The frequency of MC occurrence was MC-LA > LR > RR > D-Asp(3)-LR > YR > HilR > WR > D-Asp(3)-RR > HtyR > LY = LW = LF, while MC-RR had the highest concentrations. MCs were detected in 33 samples and 13 of these samples had more than 20% of their total MC concentration from MCs not present in US Environmental Protection Agency (US EPA) Method 544. Furthermore, seasonal deviations between the LC/MS/MS and Adda-ELISA data suggest Adda-ELISA cross-reacts with MC degradation products. This workflow provides less than 24-h turnaround for quantification and also identified key differences between LC/MS/MS and ELISA quantitation that should be investigated further.