Properties of a transplasma membrane redox system of Phanerochaete chrysosporium.
Properties of a transplasma membrane redox system of Phanerochaete chrysosporium.
复制标题
黄孢原毛平革菌跨质膜氧化还原系统的特性。
DOI：
10.1016/0003-9861(95)90021-7
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发表时间：
1995
影响因子：
3.9
通讯作者：
Aust,SD
中科院分区：
文献类型：
--
作者：
Stahl,JD;Aust,SD
研究了黄孢原毛平革菌(Phanerochaete Chrysosporium)的跨膜氧化还原体系。还原速率取决于初始铁氰化物浓度和菌丝体质量。在pH值为8.0、铁氰化钾浓度为10 mM的条件下，菌丝体的比活力为12±2nmol/min/mg(干重)。在营养限制(碳或氮)的情况下，活性下降。乙酰氰化物、间氯甲氧基苯肼、2，4-二硝基苯酚和叠氮化钠对还原有抑制作用，而氰化钾对还原无抑制作用。铁氰化物还原速率和质子输出速率随着真菌的pH高于生理pH而增加。加入铁氰化钠对质子的刺激作用相当于在pH为8.0时，用赫普斯缓冲液还原铁氰化钾的速率。讨论了这些发现与该真菌的最适生理pH和该真菌对污染物的代谢的相关性。
A transplasma membrane redox system of Phanerochaete chrysosporium was studied using ferricyanide, a membrane-impermeable electron acceptor. Rates of reduction were dependent upon initial ferricyanide concentration and mycelial mass. Specific activities of 12 ± 2 nmol/min/mg mycelia (dry wt) were consistently obtained using nutrient-sufficient mycelia at pH 8.0 and 10 mM ferricyanide. Upon nutrient limitation (either carbon or nitrogen), activity decreased. Reduction was inhibited by carbonyl cyanide m-chloromethoxyphenyl hydrazone, 2,4-dinitrophenol, and sodium azide but not by potassium cyanide at 100 nmol/mg mycelia. Ferricyanide reduction and proton export rates increased with pH above the physiological pH for the fungus. The stimulation in proton exported by the addition of ferricyanide was equal to the rate of ferricyanide reduced at pH 8.0 when Hepes buffer was used. The relevance of these findings with regard to the physiological pH optimum of the fungus and the metabolism of pollutants by this fungus is discussed.