Anticancer activity of Saussurea lappa extract by apoptotic pathway in KB human oral cancer cells
Anticancer activity of Saussurea lappa extract by apoptotic pathway in KB human oral cancer cells
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DOI：
10.3109/13880209.2013.792847
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发表时间：
2013-10
影响因子：
3.8
通讯作者：
Sung-Min Moon;S. J. Yun;J. Kook;Heung-Joong Kim;Mi Suk Choi;B. Park;Su-Gwan Kim;Byung-Ock Kim;S. Lee;H. Ahn;H. Chun;Do Kyung Kim;C. Kim
中科院分区：
文献类型：
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作者：
Sung-Min Moon;S. J. Yun;J. Kook;Heung-Joong Kim;Mi Suk Choi;B. Park;Su-Gwan Kim;Byung-Ock Kim;S. Lee;H. Ahn;H. Chun;Do Kyung Kim;C. Kim
摘要内容：风毛菊（菊科）在东亚被用作治疗腹痛和里急后重的传统草药。目前的研究表明，S. Lappa在多种癌细胞中具有抗癌活性。然而，S. lappa对口腔癌的作用及其作用机制尚未阐明。目的：探讨其对人口腔癌细胞的潜在化疗作用及细胞生长抑制机制。材料与方法：采用干燥根为材料。在本研究中使用了Lappa。用30 μg/ml的S.拉帕。为了了解其对细胞死亡的影响是否与凋亡途径有关，我们进行了DNA片段化分析，蛋白质印迹，caspase活性测定和荧光激活细胞分选（FACS）分析。结果：治疗S.在KB细胞上的拉帕提取物显著降低细胞活力，IC 50值为30 μg/ml。在处理24 h开始观察到DNA梯状条带的形成。在Western blotting分析中，S.拉帕提取物诱导caspase-3，-9和聚（ADP-核糖）聚合酶的蛋白水解加工，Bax的显着增加和Bcl-2的显着减少。我们还通过荧光显微镜证实了在活KB细胞中caspase-3/-7的活化。结论：上述结果提示S.拉帕提取物通过凋亡途径抑制KB人口腔癌细胞的增殖。
Abstract Context: Saussurea lappa Dence (Compositae) is used as a traditional herbal medicine to treat abdominal pain and tenesmus in East Asia. Current studies have shown that S. lappa has anticancer activity in divergent of cancer cells. However, the effects of S. lappa on oral cancer and its mechanisms of action have yet to be elucidated. Objective: To explore its potential chemotherapeutic effects and mechanism of cell growth inhibition on human oral cancer cells. Materials and methods: The dried roots of S. lappa were used in this study. Cell viability of KB cells was evaluated by 3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide assay after treatment with 30 µg/ml of methanol extract from the dried roots of S. lappa. To understand whether its effect on cell death is related with apoptosis pathway, we performed DNA fragmentation assay, western blot, caspase activity assay and fluorescence-activated cell sorting (FACS) analysis. Results: Treatment of S. lappa extract onto KB cells reduced cell viability significantly with an IC50 value of 30 µg/ml. The formation of a DNA ladder was observed starting at the 24 h treatment. In western blotting analysis, the S. lappa extract induced the proteolytic processing of caspase-3, -9 and poly (ADP-ribose) polymerase, a significant increase of Bax and marked reduction of Bcl-2. We also confirmed the activation of caspase-3/-7 in living KB cells by fluorescence microscopy. Conclusion: These results suggested that S. lappa extract inhibited cell proliferation through the apoptosis pathway in KB human oral cancer cells.