Embryo-induced transcriptome changes in bovine endometrium reveal species-specific and common molecular markers of uterine receptivity
Embryo-induced transcriptome changes in bovine endometrium reveal species-specific and common molecular markers of uterine receptivity
复制标题
DOI：
10.1530/rep.1.00996
复制
发表时间：
2006-08-01
期刊：
影响因子：
3.8
通讯作者：
Wolf, Eckhard
中科院分区：
文献类型：
--
作者：
Bauersachs, Stefan;Ulbrich, Susanne E.;Wolf, Eckhard
子宫内膜在早期胚胎-母体交流和妊娠的背景下在生殖组织中起着中心作用。本研究调查了妊娠第18天与非妊娠小母牛的子宫内膜样品的转录组谱，以深入了解调节子宫内膜用于胚胎附着和植入的分子机制。使用消减cDNA文库和cDNA阵列杂交的组合，在妊娠动物的子宫内膜中鉴定出109种具有至少两倍高丰度的mRNA，并且在对照组中鉴定出70种具有较高水平的mRNA。在妊娠动物中丰度较高的mRNA中，至少有41种已被描述为由干扰素诱导。此外，许多新的候选基因的转录水平参与转录，细胞粘附，调节母体免疫系统和子宫内膜重塑的调节被发现增加。用实时荧光定量PCR检测了9个基因的表达水平。通过AGRN、LGALS 3BP、LGALS 9、USP 18、PARP 12和BST 2的原位杂交显示子宫内膜中mRNA表达的定位。与在人类、小鼠中进行的类似研究进行比较，揭示了子宫容受性的物种特异性和共同的分子标志物。
The endometrium plays a central role among the reproductive tissues in the context of early embryo-maternal communication and pregnancy. This study investigated transcriptome profiles of endometrium samples from day 18 pregnant vs non-pregnant heifers to get insight into the molecular mechanisms involved in conditioning the endometrium for embryo attachment and implantation. Using a combination of subtracted cDNA libraries and cDNA array hybridisation, 109 mRNAs with at least twofold higher abundance in endometrium of pregnant animals and 70 mRNAs with higher levels in the control group were identified. Among the mRNAs with higher abundance in pregnant animals, at least 41 are already described as induced by interferons. In addition, transcript levels of many new candidate genes involved in the regulation of transcription, cell adhesion, modulation of the maternal immune system and endometrial remodelling were found to be increased. The different expression level was confirmed with real-time PCR for nine genes. Localisation of mRNA expression in the endometrium was shown by in situ hybridisation for AGRN, LGALS3BP, LGALS9, USP18, PARP12 and BST2. A comparison with similar studies in humans, mice, and revealed species-specific and common molecular markers of uterine receptivity.