EFFECT OF LOCAL ANESTHETICS ON TRANSMURAL POTENTIAL DIFFERENCE OF SMALL INTESTINE OF RAT
EFFECT OF LOCAL ANESTHETICS ON TRANSMURAL POTENTIAL DIFFERENCE OF SMALL INTESTINE OF RAT
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DOI：
10.1139/y69-079
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发表时间：
1969-01-01
影响因子：
2.1
通讯作者：
BECK, IT
中科院分区：
文献类型：
--
作者：
DINDA, PK;BECK, IT
据推测，肠跨壁电位差（PD）取决于Na + 和葡萄糖的主动转运。由于已知局部麻醉药会干扰 Na+ 和 K+ 通过神经和红细胞膜的运动，因此研究了局部麻醉药对 PD 的影响。描述了一种用于体外研究大鼠小肠透壁 PD 和吸收的装置。在该系统中，分离的肠道可保持活力至少 90 分钟。盐酸地布卡因 (0.04%) 和盐酸丁卡因 (0.15%) 抑制粘膜表面的负极性。普鲁卡因 (1.0%) 进一步抑制并最终将 PD 逆转为正极性。在所使用的浓度中，普鲁卡因增加了粘膜溶液的渗透压。在对照制剂中，通过添加甘露醇实现了类似的渗透压增加，也导致PD下降。人们认为普鲁卡因产生的总效应是药物渗透压和药理作用的总和。讨论了 Na+ 和葡萄糖转运与局麻药对 PD 抑制作用之间的可能关系。
It has been postulated that intestinal transmural potential difference (PD) depends on active transport of Na+and glucose. Since local anesthetics are known to interfere with Na+and K+movement through nerve and red cell membranes, the effect of local anesthetics on PD was investigated. An apparatus for in vitro study of transmural PD and absorption in the small intestine of rats was described. In this system the isolated gut was found to remain viable for at least 90 min. Dibucaine HCl (0.04%) and tetracaine HCl (0.15%) depressed the negative polarity of the mucosal surface. Procaine (1.0%) depressed further and finally reversed the PD to a positive polarity. In the concentration used, procaine increased the osmolarity of the mucosal solution. In the control preparation a similar increase in osmolarity, achieved by the addition of mannitol, also resulted in a fall of PD. It is thought that the total effect produced by procaine was the sum of the osmolar and pharmacological actions of the drug. A possible relationship between Na+and glucose transport in relation to the depressing effect of local anesthetics on the PD was discussed.