Pathways and biomarkers of marbling and carcass fat deposition in bovine revealed by a combination of gel-based and gel-free proteomic analyses
Pathways and biomarkers of marbling and carcass fat deposition in bovine revealed by a combination of gel-based and gel-free proteomic analyses
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DOI：
10.1016/j.meatsci.2019.05.018
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发表时间：
2019-10-01
期刊：
影响因子：
7.1
通讯作者：
Bonnet, Muriel
中科院分区：
文献类型：
--
作者：
Bazile, Jeanne;Picard, Brigitte;Bonnet, Muriel
脂肪组织（AT）沉积，肌肉内（即，大理石花纹）或胴体水平，是牛肉产业的主要经济驱动力。为了确定生物标志物和解开AT沉积的机制，我们结合了基于凝胶和无凝胶的方法，我们从不同的胴体和肌肉肥胖（CMA）的两个水平的奶牛中挖掘出胸最长肌的差异蛋白质组。我们确定了47个蛋白质的丰度，根据CMA的变化。我们列出了26个新的大理石花纹候选生物标志物，并确认了文献中已经提出的21个蛋白质。在高脂组中，有7种蛋白质参与糖酵解或脂肪异生，而有14种蛋白质参与氧化代谢、慢型肌肉或维甲酸代谢。我们强调了八种蛋白质的差异丰富和相关的CMA，从而提供了第一个清单的推定的生物标志物的尸体肥胖。这些蛋白质将在未来的研究中成为目标，旨在对牛的肥胖症进行分类。
Adipose tissue (AT) deposits, either intramuscular (i.e., marbling) or at the carcass level, are major economic drivers in the beef industry. To identify biomarkers and unravel mechanisms of AT deposition, we combined gel-based and gel-free methods and we mined the differential proteome of the longissimus thoracis from cows differing by two levels of both carcass and muscular adiposities (CMA). We identified 47 proteins with abundances that varied according to CMA. We listed 26 new candidate biomarkers of marbling and confirmed 21 proteins already proposed in the literature. Seven proteins involved in glycolysis or gluconeogenesis were the least abundant, while 14 proteins related to oxidative metabolism, slow-type muscle or retinoic acid metabolism were the most abundant in the high adiposity group. We highlighted eight proteins as differentially abundant and correlated with both CMA, thereby providing the first list of putative biomarkers of carcass adiposity. These proteins would be targeted in future studies aiming to categorize adiposity in cattle.