Fabrication of Electrochemical-DNA Biosensors for the Reagentless Detection of Nucleic Acids, Proteins and Small Molecules
Fabrication of Electrochemical-DNA Biosensors for the Reagentless Detection of Nucleic Acids, Proteins and Small Molecules
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DOI：
10.3791/2922
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发表时间：
2011-06-01
影响因子：
1.2
通讯作者：
Plaxco, Kevin W.
中科院分区：
文献类型：
--
作者：
Rowe, Aaron A.;White, Ryan J.;Plaxco, Kevin W.
在目前的医学实践中，医生将标本送到中心实验室进行检测，因此必须等待数小时或数天才能收到结果。对许多病人来说，快速的床边检查会更好。为此，我们的实验室和其他实验室开发了一种通用的无试剂生物传感器平台，支持核酸（DNA， RNA），蛋白质（包括抗体）和小分子分析物的定量，无试剂电化学检测，直接在未处理的临床和环境样品中。在这个视频中，我们演示了在这个“E-DNA”类中几种生物传感器的制备和使用。特别是，我们制造并演示了用于检测聚合酶链反应混合物、hiv特异性抗体和毒品可卡因中的目标DNA序列的传感器。准备过程只需要三个小时的动手工作，然后是一夜的孵育，它们的使用只需要几分钟。
As medicine is currently practiced, doctors send specimens to a central laboratory for testing and thus must wait hours or days to receive the results. Many patients would be better served by rapid, bedside tests. To this end our laboratory and others have developed a versatile, reagentless biosensor platform that supports the quantitative, reagentless, electrochemical detection of nucleic acids (DNA, RNA), proteins (including antibodies) and small molecules analytes directly in unprocessed clinical and environmental samples. In this video, we demonstrate the preparation and use of several biosensors in this "E-DNA" class. In particular, we fabricate and demonstrate sensors for the detection of a target DNA sequence in a polymerase chain reaction mixture, an HIV-specific antibody and the drug cocaine. The preparation procedure requires only three hours of hands-on effort followed by an overnight incubation, and their use requires only minutes.