Generation and Infection of Organotypic Cultures with Epstein-Barr Virus.
Generation and Infection of Organotypic Cultures with Epstein-Barr Virus.
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EB 病毒器官型培养物的产生和感染。
DOI：
10.1007/978-1-4939-6655-4_4
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发表时间：
2017
期刊：
影响因子：
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通讯作者：
Sample,ClareE
中科院分区：
文献类型：
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作者：
Temple,RachelM;Meyers,Craig;Sample,ClareE
虽然有许多模型系统可用于研究eb病毒在B细胞中的潜伏期，并极大地有助于我们了解这些细胞在病毒生命周期中的作用，但在体外研究上皮细胞中eb病毒生命周期的模型尚缺乏。上皮细胞在体外感染较差，ebv感染的细胞系尚未成功地从上皮肿瘤中获得。最近，我们已经证明，口腔角质形成细胞的器官型培养可以作为研究EBV感染上皮组织的模型。这些“筏形”培养产生类似于活体上皮的层状组织，具有增殖的基底层和分化的基上层。在这里，我们描述了从原代口腔粘膜上皮细胞建立的EBV感染筏培养物的一代，其表现出由分化诱导的高水平的生产性复制，以及分析EBV感染的方法。
While numerous model systems are available to study EBV latency in B cells and have contributed greatly to our understanding of the role of these cells in the viral life cycle, models to study the EBV life cycle in epithelial cells in vitro are lacking. Epithelial cells are poorly infected in vitro, and EBV-infected cell lines have not been successfully obtained from epithelial tumors. Recently, we have demonstrated that organotypic cultures of oral keratinocytes can be used as a model to study EBV infection in the epithelial tissue. These “raft” cultures generate a stratified tissue resembling the epithelium seen in vivo with a proliferating basal layer and differentiating suprabasal layers. Here, we describe generation of EBV-infected raft cultures established from primary oral mucosal epithelial cells, which exhibit high levels of productive replication induced by differentiation, as well as methods to analyze EBV infection.